Seed remains of common millet from the 4th (Mongolia) and 15th (Hungary) centuries: AFLP, SSR, and mtDNA sequence recoveries

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dc.contributor.author Gyulai, G.
dc.contributor.author Humphreys, Mervyn O.
dc.contributor.author Lagler, R.
dc.contributor.author Szabo, Z.
dc.contributor.author Toth, Z.
dc.contributor.author Bittsanszky, A.
dc.contributor.author Gyulai, F.
dc.contributor.author Heszky, L. E.
dc.date.accessioned 2009-07-15T13:44:48Z
dc.date.available 2009-07-15T13:44:48Z
dc.date.issued 2006-09
dc.identifier.citation Gyulai , G , Humphreys , M O , Lagler , R , Szabo , Z , Toth , Z , Bittsanszky , A , Gyulai , F & Heszky , L E 2006 , ' Seed remains of common millet from the 4th (Mongolia) and 15th (Hungary) centuries: AFLP, SSR, and mtDNA sequence recoveries ' Seed Science Research , vol 16 , no. 3 , pp. 179-191 . , 10.1079/SSR2006251 en
dc.identifier.issn 1475-2735
dc.identifier.other PURE: 111503
dc.identifier.other dspace: 2160/2645
dc.identifier.uri http://hdl.handle.net/2160/2645
dc.description Gyulai, G., Humphreys, M. O., Lagler, R., Szabo, Z., Toth, Z., Bittsanszky, A., Gyulai, F., Heszky, L. (2006). Seed remains of common millet from the 4th (Mongolia) and 15th (Hungary) centuries: AFLP, SSR, and mtDNA sequence recoveries. Seed Science Research, 16, (3), 179-191. Keywords: ancient DNA, excavated seeds, Panicum miliaceum Sponsorship: Hungarian Scientific Research Group OTKA-M-04 563, Organization for Economic Co-operation and Development grant OECD-JA00018642, the USA–Hungarian Fulbright Commission, and a Sze´chenyi Fellowship (SzO- 4/2003). en
dc.description.abstract Seed remains of common millet (Panicum miliaceum L.) were excavated from sites of AD 4th-century Darhan (Mongolia), and AD 15th-century Budapest (Hungary). Because the 15th-century medieval grains looked so intact, a germination test was carried out under aseptic conditions, which resulted in swelling of the grains but no cell proliferation or germination. Ancient DNA (aDNA) was extracted from the aseptic grains; analysed for amplified fragment length polymorphisms (AFLP), simple sequence repeats (SSR) and mitochondrial DNA (mtDNA); and compared with the modern millet cultivar ‘Topaz’. AFLP analysis revealed that extensive DNA degradation had occurred in the 4th-century ancient millet, resulting in only 2 (1.2%) AFLP fragments (98.8% degradation) amplified by MseCAA–EcoAGT, compared to the 15th-century medieval millet, with 158 (40%) fragments (60% degradation), and modern millet cultivar ‘Topaz’ with 264 fragments (100%). EcoAGT–Mse- CAA was found to be the most effective selectiveprimer combination for the analysis of medieval and modern millet. Eight AFLP fragments were sequenced after re-amplification and cloning. Microsatellite (SSR) analysis at the nuclear gln4, sh1, rps28 and rps15 loci revealed one SNP (single nucleotide polymorphism) at the 29th position (A ! G) of rps28 locus, compared to modern millet. An mtDNA fragment (MboI), amplified at the 18S–5S ribosomal DNA (rDNA) locus in the medieval millet, showed no molecular changes compared to modern millet. The results underline the significance of aDNA extraction and analysis of excavated seeds for comparative analysis and molecular reconstruction of ancient and extinct plant genotypes. en
dc.format.extent 13 en
dc.language.iso eng
dc.relation.ispartof Seed Science Research en
dc.subject ancient DNA en
dc.subject excavated seeds en
dc.subject Panicum miliaceum en
dc.title Seed remains of common millet from the 4th (Mongolia) and 15th (Hungary) centuries: AFLP, SSR, and mtDNA sequence recoveries en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1079/SSR2006251
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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