Show simple item record Gerard J. en_US F. en_US K. en_US L. en_US T. en_US M. en_US A. M. en_US Julie en_US S. en_US 2009-07-15T13:55:10Z 2009-07-15T13:55:10Z 2006 en_US
dc.identifier.citation Bishop , G J , Nagy , F , Shibata , K , Kozma-Bognar , L , Yokota , T , Szekeres , M , Szatmari , A M , Castle , J & Bancos , S 2006 , ' Diurnal regulation of the brassinosteroid-biosynthetic CPD gene in Arabidopsis ' Plant Physiology , pp. 299-309 . en_US
dc.identifier.other PURE: 111452 en_US
dc.identifier.other dspace: 2160/2647 en_US
dc.description.abstract Plant steroid hormones, brassinosteroids (BRs), are essential for normal photomorphogenesis. However, the mechanism by which light controls physiological functions via BRs is not well understood. Using transgenic plants carrying promoter-luciferase reporter gene fusions, we show that in Arabidopsis (Arabidopsis thaliana) the BR-biosynthetic CPD and CYP85A2 genes are under diurnal regulation. The complex diurnal expression profile of CPD is determined by dual, light-dependent, and circadian control. The severely decreased expression level ofCPDin phytochrome-deficient background and the red light-specific induction in wildtype plants suggest that light regulation of CPDis primarily mediated by phytochrome signaling. The diurnal rhythmicity ofCPD expression is maintained in brassinosteroid insensitive 1 transgenic seedlings, indicating that its transcriptional control is independent of hormonal feedback regulation. Diurnal changes in the expression of CPD and CYP85A2 are accompanied by changes of the endogenous BR content during the day, leading to brassinolide accumulation at the middle of the light phase.We also show that CPD expression is repressed in extended darkness in a BR feedback-dependent manner. In the dark the level of the bioactive hormone did not increase; therefore, our data strongly suggest that light also influences the sensitivity of plants to BRs. en_US
dc.format.extent 11 en_US
dc.relation.ispartof Plant Physiology en_US
dc.title Diurnal regulation of the brassinosteroid-biosynthetic CPD gene in Arabidopsis en_US
dc.contributor.pbl Aberystwyth University en_US
dc.contributor.pbl Institute of Biological, Environmental and Rural Sciences en_US

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