Diurnal regulation of the brassinosteroid-biosynthetic CPD gene in Arabidopsis

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dc.contributor.author Bishop, Gerard J.
dc.contributor.author Nagy, F.
dc.contributor.author Shibata, K.
dc.contributor.author Kozma-Bognar, L.
dc.contributor.author Yokota, T.
dc.contributor.author Szekeres, M.
dc.contributor.author Szatmari, A. M.
dc.contributor.author Castle, Julie
dc.contributor.author Bancos, S.
dc.date.accessioned 2009-07-15T13:55:10Z
dc.date.available 2009-07-15T13:55:10Z
dc.date.issued 2006
dc.identifier.citation Bishop , G J , Nagy , F , Shibata , K , Kozma-Bognar , L , Yokota , T , Szekeres , M , Szatmari , A M , Castle , J & Bancos , S 2006 , ' Diurnal regulation of the brassinosteroid-biosynthetic CPD gene in Arabidopsis ' Plant Physiology , pp. 299-309 . en
dc.identifier.issn 1532-2548
dc.identifier.other PURE: 111452
dc.identifier.other dspace: 2160/2647
dc.identifier.uri http://hdl.handle.net/2160/2647
dc.identifier.uri http://www.plantphysiol.org/cgi/reprint/141/1/299 en
dc.description Bancos, S., Szatmari, A. M., Castle, J., Kozma-Bognar, L., Shibata, K., Yokota, T., Bishop, G. J., Nagy, F., Szekeres, M. (2006). Diurnal regulation of the brassinosteroid-biosynthetic CPD gene in Arabidopsis. Plant Physiology, 14 (1), 299-309. en
dc.description.abstract Plant steroid hormones, brassinosteroids (BRs), are essential for normal photomorphogenesis. However, the mechanism by which light controls physiological functions via BRs is not well understood. Using transgenic plants carrying promoter-luciferase reporter gene fusions, we show that in Arabidopsis (Arabidopsis thaliana) the BR-biosynthetic CPD and CYP85A2 genes are under diurnal regulation. The complex diurnal expression profile of CPD is determined by dual, light-dependent, and circadian control. The severely decreased expression level ofCPDin phytochrome-deficient background and the red light-specific induction in wildtype plants suggest that light regulation of CPDis primarily mediated by phytochrome signaling. The diurnal rhythmicity ofCPD expression is maintained in brassinosteroid insensitive 1 transgenic seedlings, indicating that its transcriptional control is independent of hormonal feedback regulation. Diurnal changes in the expression of CPD and CYP85A2 are accompanied by changes of the endogenous BR content during the day, leading to brassinolide accumulation at the middle of the light phase.We also show that CPD expression is repressed in extended darkness in a BR feedback-dependent manner. In the dark the level of the bioactive hormone did not increase; therefore, our data strongly suggest that light also influences the sensitivity of plants to BRs. en
dc.format.extent 11 en
dc.language.iso eng
dc.relation.ispartof Plant Physiology en
dc.title Diurnal regulation of the brassinosteroid-biosynthetic CPD gene in Arabidopsis en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.contributor.institution Aberystwyth University en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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