Horizontal gene transfer from bacteria to rumen ciliates indicates adaptation to their anaerobic carbohydrates-rich environment

H...............H

Show simple item record

dc.contributor.author Nagamine, T.
dc.contributor.author Michalowski, T.
dc.contributor.author Newbold, C. James
dc.contributor.author Ricard, G.
dc.contributor.author Dutilh, B. E.
dc.contributor.author McEwan, Neil R.
dc.contributor.author Jouany, Jean-Pierre
dc.contributor.author McIntosh, Freda M.
dc.contributor.author Mitsumori, M.
dc.contributor.author al, et
dc.contributor.author Macheboeuf, Didier
dc.date.accessioned 2009-07-15T14:53:18Z
dc.date.available 2009-07-15T14:53:18Z
dc.date.issued 2009-07-15
dc.identifier.citation Nagamine , T , Michalowski , T , Newbold , C J , Ricard , G , Dutilh , B E , McEwan , N R , Jouany , J-P , McIntosh , F M , Mitsumori , M , al & Macheboeuf , D 2009 , ' Horizontal gene transfer from bacteria to rumen ciliates indicates adaptation to their anaerobic carbohydrates-rich environment ' Ricard . en
dc.identifier.other PURE: 120799
dc.identifier.other dspace: 2160/2662
dc.identifier.uri http://hdl.handle.net/2160/2662
dc.description Ricard, G., McEwan, N. R., Dutilh, B. E., Jouany, J. P., Macheboeuf, D., Mitsumori, M., McIntosh, F. M., Michalowski, T., Nagamine, T., Nelson, N., Newbold, C. J., Nsabimana, E., Takenaka, A., Thomas, N. A., Ushida, K., Hackstein, J. H. P., Huynen, M. A. (2006). Horizontal gene transfer from Bacteria to rumen Ciliates indicates adaptation to their anaerobic carbohydrates-rich environment. BMC Genomics, 7, Article No. 22. en
dc.description.abstract Background The horizontal transfer of expressed genes from Bacteria into Ciliates which live in close contact with each other in the rumen (the foregut of ruminants) was studied using ciliate Expressed Sequence Tags (ESTs). More than 4000 ESTs were sequenced from representatives of the two major groups of rumen Cilates: the order Entodiniomorphida (Entodinium simplex, Entodinium caudatum, Eudiplodinium maggii, Metadinium medium, Diploplastron affine, Polyplastron multivesiculatum and Epidinium ecaudatum) and the order Vestibuliferida, previously called Holotricha (Isotricha prostoma, Isotricha intestinalis and Dasytricha ruminantium). Results A comparison of the sequences with the completely sequenced genomes of Eukaryotes and Prokaryotes, followed by large-scale construction and analysis of phylogenies, identified 148 ciliate genes that specifically cluster with genes from the Bacteria and Archaea. The phylogenetic clustering with bacterial genes, coupled with the absence of close relatives of these genes in the Ciliate Tetrahymena thermophila, indicates that they have been acquired via Horizontal Gene Transfer (HGT) after the colonization of the gut by the rumen Ciliates. Conclusion Among the HGT candidates, we found an over-representation (>75%) of genes involved in metabolism, specifically in the catabolism of complex carbohydrates, a rich food source in the rumen. We propose that the acquisition of these genes has greatly facilitated the Ciliates' colonization of the rumen providing evidence for the role of HGT in the adaptation to new niches. en
dc.language.iso eng
dc.relation.ispartof Ricard en
dc.title Horizontal gene transfer from bacteria to rumen ciliates indicates adaptation to their anaerobic carbohydrates-rich environment en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search Cadair


Advanced Search

Browse

My Account