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dc.contributor.author Kanaya, T.
dc.contributor.author Ohwada, S.
dc.contributor.author Rose, Michael T.
dc.contributor.author al, et
dc.contributor.author Tahara, K.
dc.contributor.author Minashima, T.
dc.contributor.author Miyazawa, K.
dc.contributor.author Aso, Hisashi
dc.contributor.author Kitazawa, Haruki
dc.contributor.author Kido, T.
dc.contributor.author Watanabe, K.
dc.date.accessioned 2009-07-15T15:17:26Z
dc.date.available 2009-07-15T15:17:26Z
dc.date.issued 2009-07-15
dc.identifier.citation Kanaya , T , Ohwada , S , Rose , M T , al , Tahara , K , Minashima , T , Miyazawa , K , Aso , H , Kitazawa , H , Kido , T & Watanabe , K 2009 , ' Apoptotic process of porcine intestinal M cells ' Cell and Tissue Research , pp. 425-432 . en
dc.identifier.other PURE: 120938
dc.identifier.other dspace: 2160/2667
dc.identifier.uri http://hdl.handle.net/2160/2667
dc.description Miyazawa, K., Aso, H., Kanaya, T., Kido, T., Minashima, T., Watanabe, K., Ohwada, S., Kitazawa, H., Rose, M. T., Tahara, K., Yamasaki, T., Yamaguchi, T. (2006). Apoptotic process of porcine intestinal M cells. Cell and Tissue Research, 323, (3), 425-432. en
dc.description.abstract Membranous (M) cells of the follicle-associated epithelium (FAE) are believed to sample antigens from the gut lumen. However, the origin, differentiation mechanism, and cell death of M cells are still a matter of controversy. Therefore, we investigated the process of M cell differentiation and determined their fate in the intestine of three-way crossbred female pigs. We used anti-cytokeratin 18 and anti-PCNA antibodies to distinguish M cells and proliferative cells and performed immunohistochemistry, enzyme histochemistry, and scanning electron microscopy on fresh ileal Peyer¿s patches. Cell migration and apoptotic cells were detected by BrdU labeling and the TUNEL method, respectively. The turnover of the FAE was similar to that of the villi. M cells were mostly observed from the FAE crypt to the FAE periphery, but not in the FAE apex. As proliferative M cells (cytokeratin 18+/PCNA+ cells) have previously been detected in the FAE crypt, porcine M cells may be directly derived from intestinal epithelial stem cells and committed as a distinct cell lineage in the crypts. M cells from the FAE periphery were unstained or only weakly stained for alkaline phosphatase, whereas cytokeratin 18+/alkaline phosphatase+ cells lying near to the FAE apex showed a columnar shape similar to that of adjacent enterocytes. These data suggest that the committed M cells differentiate to mature M cells by contact with lymphocytes at the FAE periphery, and that they trans-differentiate to enterocytes and are finally excluded near the FAE apex. en
dc.format.extent 8 en
dc.language.iso eng
dc.relation.ispartof Cell and Tissue Research en
dc.title Apoptotic process of porcine intestinal M cells en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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