Rapid characterization of microbial biodegradation pathways by FT-IR spectroscopy

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dc.contributor.author Huang, Wei E.
dc.contributor.author Hopper, David J.
dc.contributor.author Goodacre, Royston
dc.contributor.author Beckmann, Manfred
dc.contributor.author Singer, Andrew
dc.contributor.author Draper, John
dc.date.accessioned 2009-07-21T10:58:34Z
dc.date.available 2009-07-21T10:58:34Z
dc.date.issued 2006-06-05
dc.identifier.citation Huang , W E , Hopper , D J , Goodacre , R , Beckmann , M , Singer , A & Draper , J 2006 , ' Rapid characterization of microbial biodegradation pathways by FT-IR spectroscopy ' Journal of Microbiological Methods , vol 67 , no. 2 , pp. 273-280 . en
dc.identifier.other PURE: 112499
dc.identifier.other dspace: 2160/2686
dc.identifier.uri http://hdl.handle.net/2160/2686
dc.description Huang, W. E., Hopper, D., Goodacre, R., Beckmann, M., Singer, A., Draper, J. (2006). Rapid characterization of microbial biodegradation pathways by FT-IR spectroscopy. Journal of Microbiological Methods, 67, (2), 273-280. en
dc.description.abstract Fourier transform-infrared (FT-IR) spectroscopy has become an important tool for rapid analysis of complex biological samples. The infrared absorbance spectrum could be regarded as a “fingerprint” which is characteristic of biochemical substances. In this study, Pseudomonas putida NCIMB 9869 was grown with either 3,5-xylenol or m-cresol as the sole carbon source, each inducing different metabolic pathways for m-cresol biotransformation. FT-IR spectroscopy was capable of differentiating both induced cultures of P. putida NCIMB 9869 as well as the resulting biotransformation product mixtures. FT-IR spectral analysis indicated that carboxylic acids were key chemicals responsible for distinguishing the products of the two catabolic pathways. Gas chromatography–mass spectrometry (GC-MS) was performed to validate the FT-IR analysis, indicating that two carboxylic acids, 3-hydroxybenzoic acid and 2,5-dihydroxybenzoic acid, were present as m-cresol biotransformation products from 3,5-xylenol-grown cells, but were absent in m-cresol-grown cells. The ability to use FT-IR to rapidly distinguish between biotransformation product mixtures as well as differentially induced bacterial strains suggests this approach might be a valuable tool for screening large biotransformation assays for novel products and metabolic mutants. en
dc.format.extent 8 en
dc.language.iso eng
dc.relation.ispartof Journal of Microbiological Methods en
dc.title Rapid characterization of microbial biodegradation pathways by FT-IR spectroscopy en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1016/j.mimet.2006.04.009
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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