The rapid differentiation of Streptomyces isolates using Fourier transform infrared spectroscopy

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dc.contributor.author Griffith, Gareth W.
dc.contributor.author Ellis, David I.
dc.contributor.author Parry, Rachel L.
dc.contributor.author Goodacre, Royston
dc.contributor.author Zhao, Hongjuan J.
dc.date.accessioned 2009-07-21T15:01:21Z
dc.date.available 2009-07-21T15:01:21Z
dc.date.issued 2009-07-21
dc.identifier.citation Griffith , G W , Ellis , D I , Parry , R L , Goodacre , R & Zhao , H J 2009 , ' The rapid differentiation of Streptomyces isolates using Fourier transform infrared spectroscopy ' 40 , pp. 213-218 . en
dc.identifier.other PURE: 121847
dc.identifier.other dspace: 2160/2699
dc.identifier.uri http://hdl.handle.net/2160/2699
dc.description Zhao, H. J., Parry, R. L., Ellis, D. I., Griffith, G. W., Goodacre, R. (2006). The rapid differentiation of Streptomyces isolates using Fourier transform infrared spectroscopy 40, (2), 213-218. Keywords: 16S rDNA sequencing; Colour grouping; FT-IR; Streptomyces en
dc.description.abstract Fifteen putative Streptomyces spp. isolated from soil were selected to be analysed using Fourier transform infrared (FT-IR) spectroscopy and 16S rRNA gene sequencing. Four colour groupings (groups 1¿4) were obtained and described according to the colour of their substrate mycelia, aerial mycelia, spore mass and pigmentation. The dendrogram constructed using unsupervised cluster analysis of the FT-IR data was in good congruence with the four colour groups and the neighbour-joining phylogenetic tree for 16S rDNA sequencing. In particular, those isolates having 100% 16S rDNA similarities which are supposed to be from the same species can be separated from each other using FT-IR analysis. This high throughput method only takes 1¿10 s to collect a FT-IR spectrum from each sample, and both 96- and 384-well microplates are available for automated analysis. FT-IR therefore presents itself as a rapid, whole-organism fingerprinting approach which can be used for preliminary differentiation of Streptomyces spp. at sub-species or strain level. en
dc.format.extent 6 en
dc.language.iso eng
dc.relation.ispartof 40 en
dc.title The rapid differentiation of Streptomyces isolates using Fourier transform infrared spectroscopy en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.contributor.institution Department of Information Studies en
dc.description.status Peer reviewed en


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