Show simple item record Whittaker, D. J. Elborough, K. M. Wilcox, P. L. Winz, R. A. Gardner, R. C. Gill, G. P. Bickerstaff, P. Humphreys, Mervyn O. Echt, C. E. Kent, J. 2009-08-05T10:25:34Z 2009-08-05T10:25:34Z 2005-11-28
dc.identifier.citation Whittaker , D J , Elborough , K M , Wilcox , P L , Winz , R A , Gardner , R C , Gill , G P , Bickerstaff , P , Humphreys , M O , Echt , C E & Kent , J 2005 , ' A framework linkage map of perennial ryegrass based on SSR markers ' Genome , pp. 354-364 . DOI: 10.1139/G05-120 en
dc.identifier.issn 1480-3321
dc.identifier.other PURE: 114042
dc.identifier.other PURE UUID: 3e7481a2-bf76-4920-a982-3fee4d3f6ea4
dc.identifier.other dspace: 2160/2772
dc.description Gill, G. P., Wilcox, P. L., Whittaker, D. J., Winz, R. A., Bickerstaff, P., Echt, C. E., Kent, J., Humphreys, M. O., Elborough, K. M., Gardner, R. C. (2006). A framework linkage map of perennial ryegrass based on SSR markers. Genome, 49, (4), 354-364. en
dc.description.abstract A moderate-density linkage map for Lolium perenne L. has been constructed based on 376 simple sequence repeat (SSR) markers. Approximately one third (124) of the SSR markers were developed from GeneThresher® libraries that preferentially select genomic DNA clones from the gene-rich unmethylated portion of the genome. The remaining SSR marker loci were generated from either SSR-enriched genomic libraries (247) or ESTs (5). Forty-five percent of the GeneThresher SSRs were associated with an expressed gene. Unlike EST-derived SSR markers, GeneThresher SSRs were often associated with genes expressed at a low level, such as transcription factors. The map constructed here fulfills 2 definitions of a 'framework map'. Firstly, it is composed of codominant markers to ensure map transferability either within or among species. Secondly, it was constructed to achieve a level of statistical confidence in the support-for-order of marker loci. The map consists of 81 framework SSR markers spread over 7 linkage groups, the same as the haploid chromosome number. Most of the remaining 295 SSR markers have been placed into their most likely interval on the framework map. Nine RFLP markers and 1 SSR marker from another map constructed using the same pedigree were also incorporated to extend genome coverage at the terminal ends of 5 linkage groups. The final map provides a robust framework with which to conduct investigations into the genetic architecture of trait variation in this commercially important grass species. en
dc.format.extent 11 en
dc.language.iso eng
dc.relation.ispartof Genome en
dc.rights en
dc.title A framework linkage map of perennial ryegrass based on SSR markers en
dc.type /dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en

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