RT-PCR analysis and stress response capacity of transgenic gshI-poplar clones (Populus x canescens) in response to paraquat exposure

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dc.contributor.author Gyulai, G.
dc.contributor.author Gullner, G.
dc.contributor.author Kiss, J.
dc.contributor.author Lagler, R.
dc.contributor.author Szabo, Z.
dc.contributor.author Bittsanszky, A.
dc.contributor.author Humphreys, Mervyn O.
dc.contributor.author al, et
dc.contributor.author Rennenberg, H.
dc.contributor.author Csintalan, Z.
dc.contributor.author Toth, G.
dc.date.accessioned 2009-08-10T09:50:09Z
dc.date.available 2009-08-10T09:50:09Z
dc.date.issued 2006
dc.identifier.citation Gyulai , G , Gullner , G , Kiss , J , Lagler , R , Szabo , Z , Bittsanszky , A , Humphreys , M O , al , E , Rennenberg , H , Csintalan , Z & Toth , G 2006 , ' RT-PCR analysis and stress response capacity of transgenic gshI-poplar clones (Populus x canescens) in response to paraquat exposure ' Zeitschrift fur Naturforschung C , pp. 699-703 . en
dc.identifier.other PURE: 109988
dc.identifier.other dspace: 2160/2808
dc.identifier.uri http://hdl.handle.net/2160/2808
dc.identifier.uri http://www.znaturforsch.com/ac/v61c/61c0699.pdf en
dc.description Bittsanszky, A., Gyulai, G., Humphreys, M. O., Gullner, G., Csintalan, Z., Kiss, J., Szabo, Z, Lagler, R., Toth, G., Rennenberg, H., Heszky, L., Komives, T. (2006). RT-PCR analysis and stress response capacity of transgenic gshI-poplar clones (Populus x canescens) in response to paraquat exposure. Zeitschrift fur Naturforschung C, 61, 699-703. en
dc.description.abstract Stress response capacity (Fv/Fm at 690 nm and F690/F735 at Fmax) ofuntransf ormed hybrid poplar, Populus x canescens (P. tremula x P. alba), and two transgenic lines overexpressing γ-ECS (γ-glutamylcysteine synthetase) either in the cytosol (cyt-ECS) or in the chloroplast (chl-ECS) was studied in response to the herbicide paraquat (4.0 x 10Ð9 to 4.0 x 10Ð6 m) for 21 days. Significant differences at sublethal (4.0 x 10Ð7 m) and bleaching (4.0 x 10Ð6 m) concentrations of paraquat were observed with about a two-fold and eight-fold decrease in the photosynthetic activity (Fv/Fm at 690 nm and F690/F735 at Fmax), respectively. None of the gshI transgenic lines (cyt-ECS, chl-ECS) with elevated GSH content exhibited significant tolerance to paraquat. Semiquantitative RT-PCR ofthe cyt-ECS clone was used for gene expression analysis of the nuclear encoded rbcS gene and the stress responsive gst gene. Expression ofthe constitutively expressed 26SrRNA ribosomal gene was probed as a control for all RT-PCR reactions. The relative intensities ofgene expressions normalized to the level of 26SrRNA intensity showed a 50% decrease in the nuclear encoded rbcS expression and a 120% increase in the stress responsive gst gene expression ofthe paraquat treated (4.0 x 10Ð7 m) samples ofthe transgenic poplar line (cyt-ECS). en
dc.format.extent 5 en
dc.language.iso eng
dc.relation.ispartof Zeitschrift fur Naturforschung C en
dc.title RT-PCR analysis and stress response capacity of transgenic gshI-poplar clones (Populus x canescens) in response to paraquat exposure en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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