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dc.contributor.author Komatsu, T.
dc.contributor.author Rose, Michael T.
dc.contributor.author Kobayashi, T.
dc.contributor.author Obara, Y.
dc.contributor.author Hagino, A.
dc.contributor.author Sakamoto, K.
dc.contributor.author Aso, Hisashi
dc.date.accessioned 2009-09-02T14:17:56Z
dc.date.available 2009-09-02T14:17:56Z
dc.date.issued 2009-09-02
dc.identifier.citation Komatsu , T , Rose , M T , Kobayashi , T , Obara , Y , Hagino , A , Sakamoto , K & Aso , H 2009 , ' Growth hormone acts on the synthesis and secretion of alpha-casein in bovine mammary epithelial cells ' Journal of Dairy Research , pp. 264-270 . en
dc.identifier.other PURE: 124339
dc.identifier.other dspace: 2160/2892
dc.identifier.uri http://hdl.handle.net/2160/2892
dc.description Sakamoto, K., Komatsu, T., Kobayashi, T., Rose, M. T., Aso, H., Hagino, A., Obara, Y. (2005). Growth hormone acts on the synthesis and secretion of alpha-casein in bovine mammary epithelial cells. Journal of Dairy Research, 72, (3), 264-270. en
dc.description.abstract To study the effect of growth hormone (GH) on the functions of mammary epithelia, we examined the effect of GH on the synthesis and secretion of ¿-casein in a bovine mammary epithelial cell (BMEC) clonal line, which was established from a 26-d-pregnant Holstein heifer. GH receptors (GHR) were observed in the BMEC on the membrane as well as in the cytoplasm. After BMEC were plated onto cell culture inserts, GH stimulated ¿-casein release in both the presence and absence of the lactogenic hormone complex, which included dexamethasone, insulin and prolactin (DIP). DIP enhanced the effect of GH on ¿-casein release. Although ¿s1-casein mRNA expression was not detected in untreated control cells, its expression was observed in BMEC in response to the GH, DIP and GH+DIP treatments. Expression was greater for GH and GH+DIP than for just DIP. Expression of GHR mRNA was increased by DIP treatment, while the mRNA expression was little changed by GH treatment. We conclude that GH acts on BMEC and induces the expression of both the ¿-casein gene and protein. GHR gene expression was shown to be regulated by DIP and GHR. GHR may be involved in a synergic effect between GH and DIP on casein secretion. These results suggest that GH, in addition to its widely accepted homeorhetic role in vivo, also can act on the mammary parenchyma, and that the effects of GH on mammary epithelial cells could partly account for the clear galactopoietic effect of recombinant bovine GH seen in lactating dairy cows. en
dc.format.extent 7 en
dc.language.iso eng
dc.relation.ispartof Journal of Dairy Research en
dc.title Growth hormone acts on the synthesis and secretion of alpha-casein in bovine mammary epithelial cells en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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