Comparative analysis of gas production profiles obtained with buffalo and sheep ruminal fluid as the source of inoculum

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dc.contributor.author Dijkstra, Jan
dc.contributor.author France, Jim
dc.contributor.author Piccolo, V.
dc.contributor.author Lopez, Sophie
dc.contributor.author Calabro, S.
dc.contributor.author Dhanoa, M. S.
dc.date.accessioned 2009-09-07T14:49:19Z
dc.date.available 2009-09-07T14:49:19Z
dc.date.issued 2005-09-30
dc.identifier.citation Dijkstra , J , France , J , Piccolo , V , Lopez , S , Calabro , S & Dhanoa , M S 2005 , ' Comparative analysis of gas production profiles obtained with buffalo and sheep ruminal fluid as the source of inoculum ' Animal Feed Science and Technology , pp. 123-124 . en
dc.identifier.issn 0377-8401
dc.identifier.other PURE: 117283
dc.identifier.other dspace: 2160/2948
dc.identifier.uri http://hdl.handle.net/2160/2948
dc.description Calabro, S., Lopez, S., Piccolo, V., Dijkstra, J., Dhanoa, M. S., France, J. (2005). Comparative analysis of gas production profiles obtained with buffalo and sheep ruminal fluid as the source of inoculum. Animal Feed Science and Technology, 123-124,(1), 51-65. en
dc.description.abstract An in vitro study was conducted to investigate effects of inoculum source (i.e., sheep versus buffalo rumen fluid) on gas production profiles, and to evaluate the suitability of various mathematical equations to fit the profiles and provide accurate values of degradation attributes. Incubations were completed using ruminal fluid obtained from sheep or buffalos fed the same diet (600 g/kg grass hay and 400 g/kg concentrate). Kinetics of fermentation of five feeds commonly fed to ruminants, being maize silage, grass silage, wheat straw, barley grain and a mixed hay, were studied with a gas production technique using an automated pressure evaluation system (APES). Ruminal fermentation characteristics (i.e., substrate disappearance, pH and VFA production) were determined after 120 h of incubation. Five mathematical functions (i.e., exponential, France, Gompertz, logistic, Morgan) were fitted to the experimental data to estimate rate and extent of feed degradation. Model comparison was based on goodness-of-fit assessed from analysis of residual variance and Akaike's information criterion. The logistic and Morgan functions were best overall, although the goodness-of-fit attained with all models was considered acceptable. Except for the Morgan, there were small differences among models in values derived for extent of degradation. There were differences between sources of inoculum in gas production measured at intermediate times (i.e., gas volumes with buffalo rumen fluid of 133 and 164 ml/g organic matter (OM) at 24 h for grass hay and silage, respectively, were smaller than those with sheep rumen fluid of 182 and 208 ml/g OM), but not at earlier or later incubation times. As a result of this trend, shorter half times, faster fermentation rates and higher extents of degradation occurred when feeds were incubated in sheep (estimated OM degradability was 0.308 and 0.402 g/g OM for grass hay and silage, respectively) compared with buffalo rumen fluid (0.246 and 0.330 g/g OM). Differences were larger for more fibrous substrates (i.e., grass silage, straw and hay) and negligible for barley grain. en
dc.format.extent 2 en
dc.language.iso eng
dc.relation.ispartof Animal Feed Science and Technology en
dc.title Comparative analysis of gas production profiles obtained with buffalo and sheep ruminal fluid as the source of inoculum en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1016/j.anifeedsci.2005.04.039
dc.contributor.institution Aberystwyth University en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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