Seven Lotus japonicus genes required for transcriptional reprogramming of the root during fungal and bacterial symbiosis

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dc.contributor.author Kistner, Catherine
dc.contributor.author Winzer, Thilo
dc.contributor.author Pitzschke, Andrea
dc.contributor.author Mulder, Lonneke
dc.contributor.author Sato, Shusei
dc.contributor.author Kaneko, Takakazu
dc.contributor.author Tabata, Satoshi
dc.contributor.author Sandal, Niels
dc.contributor.author Stougaard, Jens
dc.contributor.author Webb, K. Judith
dc.contributor.author Szczyglowski, Krzysztof
dc.contributor.author Parniske, Martin
dc.date.accessioned 2009-09-08T08:38:13Z
dc.date.available 2009-09-08T08:38:13Z
dc.date.issued 2005-08
dc.identifier.citation Kistner , C , Winzer , T , Pitzschke , A , Mulder , L , Sato , S , Kaneko , T , Tabata , S , Sandal , N , Stougaard , J , Webb , K J , Szczyglowski , K & Parniske , M 2005 , ' Seven Lotus japonicus genes required for transcriptional reprogramming of the root during fungal and bacterial symbiosis ' Plant Cell , vol 17 , no. 8 , pp. 2217-2229 . , 10.1105/tpc.105.032714 en
dc.identifier.issn 1040-4651
dc.identifier.other PURE: 119736
dc.identifier.other dspace: 2160/2977
dc.identifier.uri http://hdl.handle.net/2160/2977
dc.description Kistner, C., Winzer, T., Pitzschke, A., Mulder, L., Sato, S., Kaneko, T., Tabata, S., Sandal, N., Stougaard, J., Webb, K. J., Szczyglowski, K., Parniske, M. (2005). Seven Lotus japonicus genes required for transcriptional reprogramming of the root during fungal and bacterial symbiosis.   Plant Cell, 17, 2217-2229 Sponsorship: This work was supported by grants from the Biotechnology and Biological Sciences Research Council. Research at the Sainsbury Laboratory is funded by the Gatsby Charitable Foundation. en
dc.description.abstract A combined genetic and transcriptome analysis was performed to study the molecular basis of the arbuscular mycorrhiza (AM) symbiosis. By testing the AM phenotype of nodulation-impaired mutants and complementation analysis, we defined seven Lotus japonicus common symbiosis genes (SYMRK, CASTOR, POLLUX, SYM3, SYM6, SYM15, and SYM24) that are required for both fungal and bacterial entry into root epidermal or cortical cells. To describe the phenotype of these mutants at the molecular level, we screened for differentiating transcriptional responses of mutant and wild-type roots by large-scale gene expression profiling using cDNA-amplified fragment length polymorphism. Two percent of root transcripts was found to increase in abundance during AM development, from which a set of AM-regulated marker genes was established. A Ser-protease (SbtS) and a Cys-protease (CysS) were also activated during root nodule development. AM-induced transcriptional activation was abolished in roots carrying mutations in common symbiosis genes, suggesting a central position of these genes in a pathway leading to the transcriptional activation of downstream genes. By contrast, AM fungus-induced gene repression appeared to be unaffected in mutant backgrounds, which indicates the presence of additional independent signaling pathways. en
dc.format.extent 13 en
dc.language.iso eng
dc.relation.ispartof Plant Cell en
dc.title Seven Lotus japonicus genes required for transcriptional reprogramming of the root during fungal and bacterial symbiosis en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1105/tpc.105.032714
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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