Show simple item record Bishop, Gerard J. Yamaguchi, Shinjiro Nomura, Takahito Yokota, Takao Kamiya, Yuji Kushiro, Tetsuo 2009-09-08T09:12:29Z 2009-09-08T09:12:29Z 2005-02-14
dc.identifier.citation Bishop , G J , Yamaguchi , S , Nomura , T , Yokota , T , Kamiya , Y & Kushiro , T 2005 , ' The last reaction producing brassinolide is catalyzed by cytochrome P-450s, CYP85A3 in tomato and CYP85A2 in Arabidopsis ' Journal of Biological Chemistry , vol 280 , no. 18 , pp. 17873-17879 . DOI: 10.1074/jbc.M414592200 en
dc.identifier.issn 1083-351X
dc.identifier.other PURE: 117924
dc.identifier.other PURE UUID: 10f49a96-acbe-4de5-942b-bc3f822a4f86
dc.identifier.other dspace: 2160/2989
dc.identifier.other DSpace_20121128.csv: row: 2288
dc.identifier.other Scopus: 22344450283
dc.identifier.other PubMed: 15710611
dc.identifier.uri en
dc.description Nomura, T., Kushiro, T., Yokota, T., Kamiya, Y., Bishop, G.J., Yamaguchi, S. (2005). The last reaction producing brassinolide is catalyzed by cytochrome P-450s, CYP85A3 in tomato and CYP85A2 in Arabidopsis.  Journal of Biological Chemistry, 280, (18), 17873-17879 Sponsorship: Biotechnology and Biological Sciences Research Council and Human Frontier Science Program Organization en
dc.description.abstract Brassinosteroids are steroidal hormones essential for the growth and development of plants. Brassinolide, the most biologically active brassinosteroid, has a seven-membered lactone ring that is formed by a Baeyer-Villiger oxidation of its immediate precursor castasterone. Despite its potential key role in controlling plant development, brassinolide synthase has not been identified. Previous work has shown that the formation of castasterone from 6-deoxocastasterone is catalyzed by members of the CYP85A family of cytochrome P450 monooxygenases. A null mutation in the tomato Dwarf (CYP85A1) gene, extreme dwarf (dx), causes severe dwarfism due to brassinosteroid deficiency, but the dx mutant still produces fruits. Here, we show that dx fruits contain brassinolide at a higher level than wild-type fruits, and that a new CYP85A gene, CYP85A3, is preferentially expressed in tomato fruits. Tomato CYP85A3 catalyzed the Baeyer-Villiger oxidation to produce brassinolide from castasterone in yeast, in addition to the conversion of 6-deoxocastasterone to castasterone. We also show that Arabidopsis CYP85A2, which was initially characterized as castasterone synthase, also has brassinolide synthase activity. Exogenous application of castasterone and brassinolide to the Arabidopsis cyp85a1/cyp85a2 double mutant suggests that castasterone can function as an active brassinosteroid, but that its conversion into brassinolide is necessary for normal vegetative development in Arabidopsis. We postulate that castasterone is the major active brassinosteroid during vegetative growth in tomato, while brassinolide may play an organ-specific role in fruit development in this species. en
dc.format.extent 7 en
dc.language.iso eng
dc.relation.ispartof Journal of Biological Chemistry en
dc.rights en
dc.title The last reaction producing brassinolide is catalyzed by cytochrome P-450s, CYP85A3 in tomato and CYP85A2 in Arabidopsis en
dc.type /dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en

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