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dc.contributor.author Gerard J. en_US
dc.contributor.author Shinjiro en_US
dc.contributor.author Takahito en_US
dc.contributor.author Takao en_US
dc.contributor.author Yuji en_US
dc.contributor.author Tetsuo en_US
dc.date.accessioned 2009-09-08T09:12:29Z
dc.date.available 2009-09-08T09:12:29Z
dc.date.issued 2005-02-14 en_US
dc.identifier http://dx.doi.org/10.1074/jbc.M414592200 en_US
dc.identifier.citation Bishop , G J , Yamaguchi , S , Nomura , T , Yokota , T , Kamiya , Y & Kushiro , T 2005 , ' The last reaction producing brassinolide is catalyzed by cytochrome P-450s, CYP85A3 in tomato and CYP85A2 in Arabidopsis ' Journal of Biological Chemistry , vol 280 , no. 18 , pp. 17873-17879 . , 10.1074/jbc.M414592200 en_US
dc.identifier.other PURE: 117924 en_US
dc.identifier.other dspace: 2160/2989 en_US
dc.identifier.uri http://hdl.handle.net/2160/2989
dc.description.abstract Brassinosteroids are steroidal hormones essential for the growth and development of plants. Brassinolide, the most biologically active brassinosteroid, has a seven-membered lactone ring that is formed by a Baeyer-Villiger oxidation of its immediate precursor castasterone. Despite its potential key role in controlling plant development, brassinolide synthase has not been identified. Previous work has shown that the formation of castasterone from 6-deoxocastasterone is catalyzed by members of the CYP85A family of cytochrome P450 monooxygenases. A null mutation in the tomato Dwarf (CYP85A1) gene, extreme dwarf (dx), causes severe dwarfism due to brassinosteroid deficiency, but the dx mutant still produces fruits. Here, we show that dx fruits contain brassinolide at a higher level than wild-type fruits, and that a new CYP85A gene, CYP85A3, is preferentially expressed in tomato fruits. Tomato CYP85A3 catalyzed the Baeyer-Villiger oxidation to produce brassinolide from castasterone in yeast, in addition to the conversion of 6-deoxocastasterone to castasterone. We also show that Arabidopsis CYP85A2, which was initially characterized as castasterone synthase, also has brassinolide synthase activity. Exogenous application of castasterone and brassinolide to the Arabidopsis cyp85a1/cyp85a2 double mutant suggests that castasterone can function as an active brassinosteroid, but that its conversion into brassinolide is necessary for normal vegetative development in Arabidopsis. We postulate that castasterone is the major active brassinosteroid during vegetative growth in tomato, while brassinolide may play an organ-specific role in fruit development in this species. en_US
dc.format.extent 7 en_US
dc.relation.ispartof Journal of Biological Chemistry en_US
dc.title The last reaction producing brassinolide is catalyzed by cytochrome P-450s, CYP85A3 in tomato and CYP85A2 in Arabidopsis en_US
dc.contributor.pbl Aberystwyth University en_US
dc.contributor.pbl Institute of Biological, Environmental and Rural Sciences en_US


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