Show simple item record Minchin, Frank R. Bollard, Andrea L. Kingston-Smith, Alison H. 2009-09-08T11:08:56Z 2009-09-08T11:08:56Z 2005-01-12
dc.identifier.citation Minchin , F R , Bollard , A L & Kingston-Smith , A H 2005 , ' Stress-induced changes in protease composition are determined by nitrogen supply in non-nodulating white clover ' Journal of Experimental Botany , pp. 745-753 . DOI: 10.1093/jxb/eri049 en
dc.identifier.issn 1460-2431
dc.identifier.other PURE: 117849
dc.identifier.other PURE UUID: 27a3af9c-881b-45ab-ba7b-ca414a355b9d
dc.identifier.other dspace: 2160/3001
dc.identifier.other DSpace_20121128.csv: row: 2285
dc.identifier.other Scopus: 14544307565
dc.identifier.other PubMed: 15647316
dc.description Kingston-Smith, A. H., Bollard, A. L., Minchin, F. R. (2005). Stress-induced changes in protease composition are determined by nitrogen supply in non-nodulating white clover. Journal of Experimental Botany, 56, (412), 745-753. Sponsorship: BBSRC / DEFRA en
dc.description.abstract An inbreeding line of white clover has been identified which remains non-nodulated under appropriate physiological conditions and so the nitrogen concentration of the plant can be manipulated by altering the nitrate supply to the roots. Non-nodulating plants were used to test the hypothesis that acclimation to nitrogen limitation in white clover involves changes in protease activity and composition. These results indicate that acclimation to nitrogen limitation involves the realignment of constituent proteases without necessarily incurring significant changes in total protease activity. Plants grown at 2.5, 5.0, 7.5, and 10 mM nitrate showed a positive correlation between nitrate supply and foliar protein concentration. Protein profiles, revealed by Coomassie-stained SDS-PAGE, were unchanged between treatments for a given amount of protein. Serine, aspartate/metalloprotease, and two cysteine proteases were identified in the leaves. Although total protease activity per gram fresh weight was unchanged between treatments, the relative contributions of these four proteases was determined by nitrate supply. When plants were stressed further by withholding nitrate there was an increase in cysteine protease activity, but a senescence-related aspartate/metalloprotease was not visible. Hence, while protease expression in white clover leaves responded to the current and past nitrogen status of the plant, the proteases involved in remobilization during nutrient limitation were distinct from those involved during the main senescence period. It is suggested that nitrogen limitation induced an early, reversible stage of senescence in which perturbations in protease activity facilitated the degradation of non-essential proteins in order to increase the chances of plant survival or seed set. en
dc.format.extent 9 en
dc.language.iso eng
dc.relation.ispartof Journal of Experimental Botany en
dc.rights en
dc.title Stress-induced changes in protease composition are determined by nitrogen supply in non-nodulating white clover en
dc.type /dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en

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