Identification and mode of action of self-compatibility loci in Lolium perenne L

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dc.contributor.author Thorogood, Daniel
dc.contributor.author Armstead, Ian P.
dc.contributor.author Turner, Lesley B.
dc.contributor.author Humphreys, Mervyn O.
dc.contributor.author Hayward, Mike Denis
dc.date.accessioned 2009-09-24T11:25:27Z
dc.date.available 2009-09-24T11:25:27Z
dc.date.issued 2004-09-29
dc.identifier.citation Thorogood , D , Armstead , I P , Turner , L B , Humphreys , M O & Hayward , M D 2004 , ' Identification and mode of action of self-compatibility loci in Lolium perenne L ' Heredity , vol 94 , pp. 356-363 . en
dc.identifier.issn 1365-2540
dc.identifier.other PURE: 118662
dc.identifier.other dspace: 2160/3073
dc.identifier.uri http://hdl.handle.net/2160/3073
dc.description Thorogood, D., Armstead, I. P., Turner, L. B., Humphreys, M. O., Hayward, M. D. (2005). Identification and mode of action of self-compatibility loci in Lolium perenne L. Heredity, 94 (3), 356-363. Sponsorship: BBSRC en
dc.description.abstract The two-locus gametophytic incompatibility system in perennial ryegrass (Lolium perenne L.) is not always fully effective: obligate selfing of plants sieves self-compatible pollen mutants, and self-fertility becomes fixed in subsequent generations. Self-compatibility (SC) was investigated in an F2 family. In vitro self-pollinations were analysed and recorded and plants were classified as being either partially or fully compatible. Distorted segregation ratios of markers on linkage group (LG) 5 were found, which indicate the possible presence of a gametophytic SC locus. Interval linkage analysis of pollen compatibility after selfing confirmed that this distortion was due to a locus (T) analogous to the S5 locus of rye. However, even though markers in this region were, on average, less than 1 cM apart, the minimum number of plants possessing the unfavoured allele was never less than 6% for any marker locus. We proved that this was because of the presence of another SC locus, exhibiting gametophytic selection, segregating in this population and identified by interval mapping analysis of compatibility classes of in vitro self-pollinations. This locus was located on LG1, and probably corresponds to the S locus. We show that the T locus, a relic of a multilocus system, functions through interaction with the S locus: F2 segregation of incompatibility phenotypes and linked markers demonstrated that the S/t pollen genotype combination, expected to be compatible on selfing, was sometimes incompatible. Further evidence is presented to show that this interaction must be dependent on yet another locus located on LG2. A prime candidate would be the Z incompatibility locus. en
dc.format.extent 8 en
dc.language.iso eng
dc.relation.ispartof Heredity en
dc.title Identification and mode of action of self-compatibility loci in Lolium perenne L en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1038/sj.hdy.6800582
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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