Harpin modulates the accumulation of salicylic acid by Arabidopsis cells via apoplastic alkalization

H...............H

Show simple item record

dc.contributor.author Mur, Luis A. J.
dc.contributor.author Clarke, Andrew
dc.contributor.author Kenton, Paul
dc.contributor.author Darby, Robert M.
dc.date.accessioned 2009-09-24T12:45:43Z
dc.date.available 2009-09-24T12:45:43Z
dc.date.issued 2005-10-24
dc.identifier.citation Mur , L A J , Clarke , A , Kenton , P & Darby , R M 2005 , ' Harpin modulates the accumulation of salicylic acid by Arabidopsis cells via apoplastic alkalization ' Journal of Experimental Botany , pp. 3129-3136 . en
dc.identifier.issn 1460-2431
dc.identifier.other PURE: 119273
dc.identifier.other dspace: 2160/3076
dc.identifier.uri http://hdl.handle.net/2160/3076
dc.description Clarke, A., Mur, L. A. J., Darby, R. M., Kenton, P. (2005). Harpin modulates the accumulation of salicylic acid by Arabidopsis cells via apoplastic alkalization. Journal of Experimental Botany, 56, (422), 3129-3136. Sponsorship: Leverhulme Trust project grant F/00 424/D. en
dc.description.abstract It is reported here that salicylic acid (SA) is rapidly taken up by Arabidopsis cells, and its uptake is accompanied by media alkalization and cytosolic acidification, and it is inhibited by the ionophore nigericin, suggesting that its import is linked with that of H+ and driven by a proton gradient. Such import and accumulation declined sharply within a narrow physiological pH range (pH 5.7–6.1), corresponding to a reduction in the [H+] of the media from 1.99 µmol l–1 to 0.79 µmol l–1. Following the initial uptake, SA was exported back into the media as free SA against a continued [H+]-dependent import. Since the uptake and accumulation of SA declines sharply within a narrow pH range and cell wall alkalization is an early response during incompatible plant/pathogen interactions, the bacterial elicitor harpinPss was used to investigate how SA transport may be modulated during defence responses. Harpin induced a rapid and sustained alkalization of the cell suspension media, reaching the critical pH (pH 5.9–6.1) at which SA import is inhibited at c. 60 min. Such media alkalization corresponded with a reduction in the SA associated with cells co-treated with harpin, and an inhibition of SA uptake in cells pretreated with harpin. Scavengers of ROS, or compounds which generate H2O2 or NO had little effect on the import or net export of SA, suggesting that media alkalization induced by harpin is sufficient to modulate the kinetics of SA transport. en
dc.format.extent 8 en
dc.language.iso eng
dc.relation.ispartof Journal of Experimental Botany en
dc.title Harpin modulates the accumulation of salicylic acid by Arabidopsis cells via apoplastic alkalization en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1093/jxb/eri310
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search Cadair


Advanced Search

Browse

My Account