A gene cluster for secondary metabolism in oat: Implications for the evolution of metabolic diversity in plants

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dc.contributor.author Melton, R.
dc.contributor.author Qi, X.
dc.contributor.author Osbourn, A.
dc.contributor.author Maxwell, C.
dc.contributor.author Leggett, J. Michael
dc.contributor.author Bakht, S.
dc.date.accessioned 2009-09-24T13:18:36Z
dc.date.available 2009-09-24T13:18:36Z
dc.date.issued 2004-05-17
dc.identifier.citation Melton , R , Qi , X , Osbourn , A , Maxwell , C , Leggett , J M & Bakht , S 2004 , ' A gene cluster for secondary metabolism in oat: Implications for the evolution of metabolic diversity in plants ' Proceedings of the National Academy of Sciences of the United States of America , pp. 8233-8238 . en
dc.identifier.other PURE: 121138
dc.identifier.other dspace: 2160/3080
dc.identifier.uri http://hdl.handle.net/2160/3080
dc.description Qi, X., Bakht, S., Leggett, J. M., Maxwell, C., Melton, R., Osbourn, A. (2004). A gene cluster for secondary metabolism in oat: Implications for the evolution of metabolic diversity in plants. Proceedings of the National Academy of Sciences of the United States of America, 101, (21), 8233-8238. Sponsorship: BBSRC / The Sainsbury Laboratory en
dc.description.abstract The evolution of the ability to synthesize specialized metabolites is likely to have been key for survival and diversification of different plant species. Oats (Avena spp.) produce antimicrobial triterpenoids (avenacins) that protect against disease. The oat -amyrin synthase gene AsbAS1, which encodes the first committed enzyme in the avenacin biosynthetic pathway, is clearly distinct from other plant -amyrin synthases. Here we show that AsbAS1 has arisen by duplication and divergence of a cycloartenol synthase- like gene, and that its properties have been refined since the divergence of oats and wheat. Strikingly, we have also found that AsbAS1 is clustered with other genes required for distinct steps in avenacin biosynthesis in a region of the genome that is not conserved in other cereals. Because the components of this gene cluster are required for at least four clearly distinct enzymatic processes (2,3-oxidosqualene cyclization, -amyrin oxidation, glycosylation, and acylation), it is unlikely that the cluster has arisen as a consequence of duplication of a common ancestor. Although clusters of paralogous genes are common in plants (e.g., gene clusters for rRNA and specific disease resistance), reports of clusters of genes that do not share sequence relatedness and whose products contribute to a single selectable function are rare [Gierl, A. & Frey, M. (2001) Planta 213, 493–498]. Taken together, our evidence has important implications for the generation of metabolic diversity in plants. en
dc.format.extent 6 en
dc.language.iso eng
dc.relation.ispartof Proceedings of the National Academy of Sciences of the United States of America en
dc.title A gene cluster for secondary metabolism in oat: Implications for the evolution of metabolic diversity in plants en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1073/pnas.0401301101
dc.contributor.institution Aberystwyth University en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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