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dc.contributor.author Newbold, C. James
dc.contributor.author Travis, A. J.
dc.contributor.author Chareyron, E. N.
dc.contributor.author Nelson, Nancy
dc.contributor.author Calza, R. E.
dc.contributor.author Duval, S. M.
dc.contributor.author Wallace, R. John
dc.contributor.author McEwan, Neil R.
dc.contributor.author McIntosh, Freda M.
dc.contributor.author Eschenlauer, S. C. P.
dc.date.accessioned 2009-09-25T14:28:06Z
dc.date.available 2009-09-25T14:28:06Z
dc.date.issued 2009-09-25
dc.identifier.citation Newbold , C J , Travis , A J , Chareyron , E N , Nelson , N , Calza , R E , Duval , S M , Wallace , R J , McEwan , N R , McIntosh , F M & Eschenlauer , S C P 2009 , ' An NAD+-dependent glutamate dehydrogenase cloned from the ruminal ciliate protozoan, Entodinium caudatum ' Unknown Journal , pp. 113-121 . en
dc.identifier.other PURE: 128096
dc.identifier.other dspace: 2160/3094
dc.identifier.uri http://hdl.handle.net/2160/3094
dc.description Newbold, C. J., McEwan, N. R., Calza, R. E., Chareyron, E. N., Duval, S. M., Eschenlauer, S. C .P., McIntosh, F. M., Nelson, N., Travis, A. J., Wallace, R. J. (2005). An NAD+-dependent glutamate dehydrogenase cloned from the ruminal ciliate protozoan, Entodinium caudatum.  FEMS Microbiology Letters, 247, (2), 113-121 Keywords: Ammonia uptake; Entodinium caudatum; Glutamate dehydrogenase; Protozoa; Rumen en
dc.description.abstract An NAD+-dependent glutamate dehydrogenase (GDH; EC 1.4.1.24) was cloned from the ruminal ciliate protozoan, Entodinium caudatum. The gene had high sequence similarity to GDH genes from the Bacteroides (class) ¿ a class of bacteria which is highly represented in the rumen. When expressed in Escherichia coli the enzyme had a high affinity for ammonia and ¿-ketoglutarate (apparent Km of 2.33 and 0.71 mM, respectively) and a low affinity for glutamate (apparent Km of 98 mM). GDH activity and GDH mRNA concentration were increased by incubating washed E. caudatum cells with ammonia and antibiotics. These results suggest that the GDH is an anabolic enzyme catalysing the assimilation of ammonia by E. caudatum in the rumen and that the gene was probably acquired by lateral gene transfer from a ruminal bacterium. en
dc.format.extent 9 en
dc.language.iso eng
dc.relation.ispartof Unknown Journal en
dc.title An NAD+-dependent glutamate dehydrogenase cloned from the ruminal ciliate protozoan, Entodinium caudatum en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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