AFLP analysis and improved phytoextraction capacity of transgenic gshI-poplar clones (Populus x canescens ) for copper in vitro

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dc.contributor.author Skøt, Leif
dc.contributor.author Gullner, G.
dc.contributor.author Heywood, Susan
dc.contributor.author Humphreys, Mervyn O.
dc.contributor.author Bittsanszky, A.
dc.contributor.author Lovatt, J. Alan
dc.contributor.author Gyulai, G.
dc.contributor.author Abberton, Michael T.
dc.contributor.author Roderick, Hywel W.
dc.contributor.author Kiss, J.
dc.contributor.author Skøt, Kirsten P.
dc.date.accessioned 2009-09-29T15:46:29Z
dc.date.available 2009-09-29T15:46:29Z
dc.date.issued 2009-09-29
dc.identifier.citation Skøt , L , Gullner , G , Heywood , S , Humphreys , M O , Bittsanszky , A , Lovatt , J A , Gyulai , G , Abberton , M T , Roderick , H W , Kiss , J & Skøt , K P 2009 , ' AFLP analysis and improved phytoextraction capacity of transgenic gshI-poplar clones (Populus x canescens ) for copper in vitro ' Gyulai , pp. 3-4 . en
dc.identifier.other PURE: 128427
dc.identifier.other dspace: 2160/3115
dc.identifier.uri http://hdl.handle.net/2160/3115
dc.description Gyulai, G., Humphreys, M. O., Bittsanszky, A., Skøt, K. P., Kiss, J., Skøt, L., Gullner, G., Heywood, S., Szabo, Z., Lovatt, J. A., Radimszky, L., Roderick, H. W., Rennenberg, H., Abberton, M. T., Komives, T., Heszky, L. E. (2005). AFLP analysis and improved phytoextraction capacity of transgenic gshI-poplar clones (Populus x canescens) for copper in vitro.   Zeitschrift fur Naturforschung C, 60,(3-4), 300-306. en
dc.description.abstract Clone stability and in vitro phytoextraction capacity of vegetative clones of P. ¥ canescens (2n = 4x = 38) including two transgenic clones (ggs11 and lgl6) were studied as in vitro leaf disc cultures. Presence of the gshI-transgene in the transformed clones was detected in PCR reactions using gshI-specific primers. Clone stability was determined by fAFLP (fluorescent amplified DNA fragment length polymorphism) analysis. In total, 682 AFLP fragments were identified generated by twelve selective primer pairs after EcoRIÐMseI digestion. Four fragments generated by EcoAGTÐMseCCC were different (99.4% genetic similarity) which proves an unexpectedly low bud mutation frequency in P. ¥ canescens. For the study of phytoextraction capacity leaf discs (8 mm) were exposed to a concentration series of ZnSO4 (10Ð1 to 10Ð5 m) incubated for 21 days on aseptic tissue culture media WPM containing 1 ¿m Cu. Zn2+ caused phytotoxicity only at high concentrations (10Ð1 to 10Ð2 m). The transgenic poplar cyt-ECS (ggs11) clone, as stimulated by the presence of Zn, showed elevated heavy metal (Cu) uptake as compared to the non-transformed clone. These results suggest that gshI-transgenic poplars may be suitable for phytoremediation of soils contaminated with zinc and copper. en
dc.format.extent 2 en
dc.language.iso eng
dc.relation.ispartof Gyulai en
dc.title AFLP analysis and improved phytoextraction capacity of transgenic gshI-poplar clones (Populus x canescens ) for copper in vitro en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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