Morphological and molecular analysis of common millet (P. miliaceum) cultivars compared to an aDNA sample from the 15th century (Hungary)

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dc.contributor.author Holly, L.
dc.contributor.author Gyulai, G.
dc.contributor.author Humphreys, Mervyn O.
dc.contributor.author Heszky, L. E.
dc.contributor.author Horvath, L.
dc.contributor.author Szabo, Z.
dc.contributor.author Lagler, R.
dc.contributor.author Bittsanszky, A.
dc.contributor.author Kiss, J.
dc.date.accessioned 2009-10-08T08:29:01Z
dc.date.available 2009-10-08T08:29:01Z
dc.date.issued 2005
dc.identifier.citation Holly , L , Gyulai , G , Humphreys , M O , Heszky , L E , Horvath , L , Szabo , Z , Lagler , R , Bittsanszky , A & Kiss , J 2005 , ' Morphological and molecular analysis of common millet (P. miliaceum) cultivars compared to an aDNA sample from the 15th century (Hungary) ' Euphytica , pp. 1-2 . en
dc.identifier.issn 1573-5060
dc.identifier.other PURE: 115842
dc.identifier.other dspace: 2160/3171
dc.identifier.uri http://hdl.handle.net/2160/3171
dc.description Lagler, R., Gyulai, G., Humphreys, M. O., Szabo, Z., Horvaith, L., Bittsanszky, A., Kiss, J., Holly, L., Heszky, L. (2005). Morphological and molecular analysis of common millet (P. miliaceum) cultivars compared to an aDNA sample from the 15th century (Hungary). Euphytica, 146, (1-2), 77-85 Keywords: ancientDNA - CAP - mtDNA - ISSR - SNPs en
dc.description.abstract Morphological characterization of 20 common millet (Panicum miliaceum L., 2n = 4x = 36) cultivars and landraces revealed four distinct clusters which were apparently consistent with the grain colors of black, black and brown, red, yellow, and white. Seed remains of medieval millet, recovered from a 15th century layer (King's Palace, Budapest, Hungary), showed reddish yellow grain color after rehydrating on tissue culture medium that was close to grain color of modern cultivar Omszkoje. aDNA of medieval commom millet was extracted successfully, analyzed and compared to modern common millets by ISSR, SSR, CAP and mtDNA. Analyses of fragments and sequences revealed polymorphism at seven ISSR loci (15 alleles) and at the 5S-18S rDNA locus of mtDNA. CAP analysis of the 5S-18S rDNA fragment revealed no SNPs in the restriction sites of six endonucleases TaqI, BsuRI, HinfI, MboI, AluI and RsaI. Sequence alignments of the restriction fragments RsaI also revealed consensus sequence in the medieval sample compared to a modern variety. An attempted phenotype reconstruction indicated that medieval common millet showed the closest morphological similarity to modern millet cultivar Omszkoje. en
dc.format.extent 2 en
dc.language.iso eng
dc.relation.ispartof Euphytica en
dc.title Morphological and molecular analysis of common millet (P. miliaceum) cultivars compared to an aDNA sample from the 15th century (Hungary) en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1007/s10681-005-5814-7
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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