Unique and overlapping expression patterns of Arabidopsis CYP85 genes involved in brassinosteroid C-6 oxidation

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dc.contributor.author Bishop, Gerard J.
dc.contributor.author Jenkins, Gwen
dc.contributor.author Szekeres, M.
dc.contributor.author Castle, Julie
dc.date.accessioned 2009-10-08T09:15:06Z
dc.date.available 2009-10-08T09:15:06Z
dc.date.issued 2005
dc.identifier.citation Bishop , G J , Jenkins , G , Szekeres , M & Castle , J 2005 , ' Unique and overlapping expression patterns of Arabidopsis CYP85 genes involved in brassinosteroid C-6 oxidation ' Plant Molecular Biology , pp. 129-240 . en
dc.identifier.issn 1573-5028
dc.identifier.other PURE: 115760
dc.identifier.other dspace: 2160/3176
dc.identifier.uri http://hdl.handle.net/2160/3176
dc.description Castle, J., Szekeres, M., Jenkins, G., Bishop, G.J. (2005). Unique and overlapping expression patterns of Arabidopsis CYP85 genes involved in brassinosteroid C-6 oxidation. Plant Molecular Biology, 57, (1), 129-240 Keywords: brassinosteroids - CYP85 - differential expression - reporter genes - steroid hormone synthesis Sponsorship: BBSRC en
dc.description.abstract Brassinosteroids (BRs) are steroid hormones that are essential for plant growth and development. To gain insight into potential sites of BR synthesis, we studied promoter activities of the two Arabidopsis BR C-6 oxidase genes (CYP85A1 and CYP85A2) in transgenic plants carrying promoter fusions with the GUS, GFP or LUC reporter genes. BR-dependent feedback regulation of the GUS reporter constructs indicated that their expression corresponded to those of the native genes. Both the CYP85A1 and CYP85A2 promoters showed maximum activity during the first week following germination, particularly in the vascular tissues. Compared to CYP85A2, CYP85A1 expression was weaker and confined to the early stages of seedling development. Stronger CYP85A2 promoter activity was evident in both juvenile and adult plants. Comparison of the 5-UTR and TATA box sequences of CYP85A1 and CYP85A2 revealed high homology, indicating a relatively recent gene duplication. We also found that transgenic Arabidopsis plants harbouring the tomato DWARF promoter-GUS fusion had similarities in the expression pattern to the Arabidopsis genes suggesting common transcriptional regulation of CYP85 genes in the two species. en
dc.format.extent 112 en
dc.language.iso eng
dc.relation.ispartof Plant Molecular Biology en
dc.title Unique and overlapping expression patterns of Arabidopsis CYP85 genes involved in brassinosteroid C-6 oxidation en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1007/s11103-004-6851-7
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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