Magnaporthe grisea interactions with the model grass Brachypodium distachyon closely resemble those with rice (Oryza sativa)

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dc.contributor.author Routledge, A. P. M.
dc.contributor.author Draper, John H.
dc.contributor.author Talbot, N. J.
dc.contributor.author Smith, J. V.
dc.contributor.author Mur, Luis A. J.
dc.contributor.author Shelley, G.
dc.date.accessioned 2009-10-08T09:59:20Z
dc.date.available 2009-10-08T09:59:20Z
dc.date.issued 2004
dc.identifier.citation Routledge , A P M , Draper , J H , Talbot , N J , Smith , J V , Mur , L A J & Shelley , G 2004 , ' Magnaporthe grisea interactions with the model grass Brachypodium distachyon closely resemble those with rice (Oryza sativa) ' . in Molecular Plant Pathology . Blackwell Publishing , pp. 253-265 . en
dc.identifier.other PURE: 122236
dc.identifier.other dspace: 2160/3177
dc.identifier.uri http://hdl.handle.net/2160/3177
dc.description Routledge, A. P. M., Shelley, G., Smith, J. V., Talbot, N. J., Draper, J., Mur, L. A. J. (2004). Magnaporthe grisea interactions with the model grass Brachypodium distachyon closely resemble those with rice (Oryza sativa). Molecular Plant Pathology, 5, (4), 253-265. Sponsorship: BBSRC ROPA grant 2/9708842 en
dc.description.abstract Germplasm of Brachypodium distachyon was inoculated with Magnaporthe grisea using either rice- (Guy11) or grass-adapted (FAG1.1.1, PA19w-06, PA31v-01) host-limited forms of the fungus, and interactions with varying degrees of susceptibility and resistance were identified. Ecotype ABR5 was resistant to each M. grisea strain whereas ABR1 was susceptible to all but P31vi-01. Mendelian segregation in ABR1 × ABR5 crosses suggested that a single dominant resistance gene conferred resistance to Guy11. Microscopic analyses revealed that the aetiology of Guy11 fungal development and disease progression in ABR1 closely resembled that of rice infections. In ABR5, Guy11 pathogenesis was first suppressed at 48 h post-inoculation, at the secondary hyphal formation stage and was coincident with cytoplasmic granulation. Resistance to strains PA31v-01 and FAG1.1.1 was associated with a localized cell death with little callose deposition. 3,3-Diaminobenzidine staining indicated the elicitation of cell death in B. distachyon was preceded by oxidative stress in the interacting epidermal cells and the underlying mesophyll cells. Northern blot hybridization using probes for barley genes (PR1, PR5 and PAL) indicated that each was more rapidly expressed in ABR5 challenged with Guy11 although the B. distachyon defence genes BD1 and BD8 were more quickly induced in ABR1. Such data show that B. distachyon is an appropriate host for functional genomic investigations into M. grisea pathology and plant responses. en
dc.format.extent 13 en
dc.language.iso eng
dc.publisher Blackwell Publishing
dc.relation.ispartof Molecular Plant Pathology en
dc.title Magnaporthe grisea interactions with the model grass Brachypodium distachyon closely resemble those with rice (Oryza sativa) en
dc.type Text en
dc.type.publicationtype Book chapter en
dc.identifier.doi http://dx.doi.org/10.1111/j.1364-3703.2004.00224.x
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en


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