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dc.contributor.author Regensbogenova, M.
dc.contributor.author Javorsky, P.
dc.contributor.author McEwan, N. R.
dc.contributor.author Kisidayova, Svetlana
dc.contributor.author Jouany, Jean-Pierre
dc.contributor.author Michalowski, T.
dc.contributor.author Newbold, C. James
dc.contributor.author AL, ET
dc.contributor.author Moon-Van Der Staay, G. W. M.
dc.contributor.author Hackstein, J. H. P.
dc.contributor.author Moon-Van Der Staay, S. Y.
dc.date.accessioned 2009-10-08T11:08:57Z
dc.date.available 2009-10-08T11:08:57Z
dc.date.issued 2009-10-08
dc.identifier.citation Regensbogenova , M , Javorsky , P , McEwan , N R , Kisidayova , S , Jouany , J-P , Michalowski , T , Newbold , C J , AL , E T , Moon-Van Der Staay , G W M , Hackstein , J H P & Moon-Van Der Staay , S Y 2009 , ' Rapid identification of rumen protozoa by restriction analysis of amplified 18S rRNA gene ' Acta Protozoologica , pp. 219-224 . en
dc.identifier.other PURE: 131359
dc.identifier.other dspace: 2160/3188
dc.identifier.uri http://hdl.handle.net/2160/3188
dc.description Regensbogenova, M., Kisidayova, S., Michalowski, T., Javorsky, P., Moon-Van Der Staay, S. Y., Moon-Van Der Staay, G. W. M., Hackstein, J. H. P., McEwan, N. R., Jouany, J. P., Newbold, C. J., Pristas, P. (2004). Rapid identification of rumen protozoa by restriction analysis of amplified 18S rRNA gene. Acta Protozoologica, 43, 219-224. en
dc.description.abstract A rapid method has been developed for molecular identification of rumen ciliates without the need for cultivation. Total DNA was isolated from single protozoal cells by the Chelex method and nearly complete protozoal 18S rRNA genes were amplified and subjected to restriction fragment length polymorphism analysis. On the basis of restriction patterns generated a molecular key was elaborated allowing identification of protozoa solely by a molecular technique without prior knowledge of morphology. No differences were observed between identical species originating from different animals or geographic locations, or between morphological variants of the same species. The ARDREA analysis described here provides a rapid and convenient way for identification and diversity studies of rumen protozoa. en
dc.format.extent 6 en
dc.language.iso eng
dc.relation.ispartof Acta Protozoologica en
dc.title Rapid identification of rumen protozoa by restriction analysis of amplified 18S rRNA gene en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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