Show simple item record Takano, Shoichi Takasuga, Akiko Tahara, Kodi Aso, Hisashi Yamaguchi, Takahiro Yamasaki, Tadashi Rose, Michael T. Sugimoto, Yoshikazu 2009-10-17T12:18:01Z 2009-10-17T12:18:01Z 2009-10-17
dc.identifier.citation Takano , S , Takasuga , A , Tahara , K , Aso , H , Yamaguchi , T , Yamasaki , T , Rose , M T & Sugimoto , Y 2009 , ' Cloning and expression of type XII collagen isoforms during bovine adipogenesis ' Unknown Journal , pp. 113-122 . en
dc.identifier.other PURE: 132814
dc.identifier.other dspace: 2160/3236
dc.description Tahara, K., Aso, H., Yamasaki, T., Rose, M. T., Takasuga, A., Sugimoto, Y., Yamaguchi, T., Takano, S. (2004). Cloning and expression of type XII collagen isoforms during bovine adipogenesis.   Differentiation, 72, (4), 113-122 en
dc.description.abstract AbstractIn order to isolate candidate genes involved in bovine adipocyte differentiation, we have constructed a subtraction library from a clonal bovine intra-muscular pre-adipocyte (BIP) cell line using the suppression subtractive hybridization method. We have isolated a set of subtracted cDNA fragments whose respective mRNA levels are up-regulated during the adipogenic differentiation of BIP cells, and cloned cDNAs from a differentiated BIP-¿ ZAP II cDNA library. Two cDNA clones were highly homologous to the sequence of mouse and human type XII collagen ¿-1, determined by a BLAST homology search. As type XII collagen has been reported to have four types of splicing isoform, two clones were determined to be XII-1 and XII-2 splicing isoforms, respectively, because of a difference in the C-terminal NC1 domain. From the expression analysis of type XII collagen, the XIIA-2 isoform was mainly expressed in differentiated BIP cells and adipose tissues. Although the function of type XII collagen has not been established as yet, these results suggest that type XII collagen may be associated with adipocyte differentiation and adipose formation in cattle and is a potentially useful marker for adipogenesis. en
dc.format.extent 10 en
dc.language.iso eng
dc.relation.ispartof Unknown Journal en
dc.title Cloning and expression of type XII collagen isoforms during bovine adipogenesis en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en

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