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dc.contributor.author Fahy, B.
dc.contributor.author Denyer, K.
dc.contributor.author Moates, G.
dc.contributor.author Verhoeven, T.
dc.contributor.author Leggett, J. Michael
dc.date.accessioned 2009-10-19T15:18:02Z
dc.date.available 2009-10-19T15:18:02Z
dc.date.issued 2004-07
dc.identifier.citation Fahy , B , Denyer , K , Moates , G , Verhoeven , T & Leggett , J M 2004 , ' Isolation and characterisation of novel starch mutants of oats ' Journal of Cereal Science , pp. 69-79 . , 10.1016/j.jcs.2004.04.004 en
dc.identifier.other PURE: 122923
dc.identifier.other dspace: 2160/3263
dc.identifier.uri http://hdl.handle.net/2160/3263
dc.description Verhoeven, T., Fahy, B., Leggett, J. M., Moates, G., Denyer, K. (2004). Isolation and characterisation of novel starch mutants of oats. Journal of Cereal Science, 40, (1), 69-79. Sponsorship: BBSRC en
dc.description.abstract Mutagenised grains of the diploid oat, Avena strigosa Schreb., were screened for altered starch granules in the endosperm. Three mutant lines were identified: mutants lam-1 and lam-2 had granules that stained red with iodine solution and sga-1 had endosperm containing soluble material that stained red with iodine solution, as well as blue-staining starch granules. The lam-1 and lam-2 lines lack or have severely reduced amounts of granule-bound starch synthase activity, the granule-bound starch synthase I protein and the amylose component of starch in the endosperm. They therefore represent mutations of the waxy type. The red-staining, soluble material in the sga-1 mutant is very similar in appearance and chain-length profile to the phytoglycogen that accumulates in the endosperms of the sugary-1 mutants of cereals. All sugary-1 mutants lack or have reduced amounts of isoamylase activity. However, native gel assays reveal a normal isoamylase activity in the developing endosperms of sga-1 suggesting that it is not of the sugary-1 type. The nature of the mutation responsible for the sga-1 phenotype has yet to be discovered. en
dc.format.extent 11 en
dc.language.iso eng
dc.relation.ispartof Journal of Cereal Science en
dc.title Isolation and characterisation of novel starch mutants of oats en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1016/j.jcs.2004.04.004
dc.contributor.institution Aberystwyth University en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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