Influence of morphology on the near-infrared spectra of mycelial biomass and its implications in bioprocess monitoring

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dc.contributor.author Vaidyanathan, Seetharaman
dc.contributor.author Harvey, Linda M.
dc.contributor.author McNeil, Brian
dc.contributor.author White, Stweart
dc.date.accessioned 2009-11-02T12:41:44Z
dc.date.available 2009-11-02T12:41:44Z
dc.date.issued 2003-03-20
dc.identifier.citation Vaidyanathan , S , Harvey , L M , McNeil , B & White , S 2003 , ' Influence of morphology on the near-infrared spectra of mycelial biomass and its implications in bioprocess monitoring ' Biotechnology and Bioengineering , vol 82 , no. 6 , pp. 715-724 . en
dc.identifier.issn 1097-0290
dc.identifier.other PURE: 139715
dc.identifier.other dspace: 2160/3376
dc.identifier.uri http://hdl.handle.net/2160/3376
dc.description Vaidyanathan, S., White, S, Harvey, L.M., McNeil, B. (2003). Influence of morphology on the near-infrared spectra of mycelial biomass and its implications in bioprocess monitoring.   Biotechnology and Bioengineering, 82, (6), 715-724 en
dc.description.abstract Bioprocesses that employ mycelial microorganisms are commercially important. The application of optical techniques for the measurement of biomass in such processes is limited by the morphological heterogeneity exhibited by the mycelial microorganism employed. We investigated the influence of morphology on the near-infrared (NIR) spectra of the biomass of Streptomyces fradiae, a filamentous microorganism, by studying the spectra of mycelial suspensions that were manipulated to generate a range of morphological forms. Computerized image analysis was used to characterize the morphological forms. Principal component analysis was used to assess the spectral variations and study correlations to the manipulated mycelial morphology. Although morphology was found to influence the near infrared transmittance spectra of biomass, the influence was less pronounced than in the visible region, the spectral information at longer wavelengths (1600-2350 nm) showing greater stability to morphological variations. Long-wave NIR spectral information is therefore likely to be more useful in estimating biomass in mycelial bioprocesses. Furthermore, the NIR reflectance spectra of dried biomass were found to show correlations to the morphological variations introduced, suggesting that NIR spectra may be useful in obtaining morphology related information. © 2003 Wiley Periodicals, Inc. Biotechnol Bioeng 82: 715-724, 2003. en
dc.format.extent 10 en
dc.language.iso eng
dc.relation.ispartof Biotechnology and Bioengineering en
dc.subject near-infrared en
dc.subject morphology en
dc.subject mycelial en
dc.subject Streptomyces en
dc.subject bioprocess en
dc.title Influence of morphology on the near-infrared spectra of mycelial biomass and its implications in bioprocess monitoring en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1002/bit.10621
dc.contributor.institution Aberystwyth University en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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