Show simple item record Seetharaman en_US Linda M. en_US Brian en_US Stweart en_US 2009-11-02T12:41:44Z 2009-11-02T12:41:44Z 2003-03-20 en_US
dc.identifier en_US
dc.identifier.citation Vaidyanathan , S , Harvey , L M , McNeil , B & White , S 2003 , ' Influence of morphology on the near-infrared spectra of mycelial biomass and its implications in bioprocess monitoring ' Biotechnology and Bioengineering , vol 82 , no. 6 , pp. 715-724 . , 10.1002/bit.10621 en_US
dc.identifier.other PURE: 139715 en_US
dc.identifier.other dspace: 2160/3376 en_US
dc.description.abstract Bioprocesses that employ mycelial microorganisms are commercially important. The application of optical techniques for the measurement of biomass in such processes is limited by the morphological heterogeneity exhibited by the mycelial microorganism employed. We investigated the influence of morphology on the near-infrared (NIR) spectra of the biomass of Streptomyces fradiae, a filamentous microorganism, by studying the spectra of mycelial suspensions that were manipulated to generate a range of morphological forms. Computerized image analysis was used to characterize the morphological forms. Principal component analysis was used to assess the spectral variations and study correlations to the manipulated mycelial morphology. Although morphology was found to influence the near infrared transmittance spectra of biomass, the influence was less pronounced than in the visible region, the spectral information at longer wavelengths (1600-2350 nm) showing greater stability to morphological variations. Long-wave NIR spectral information is therefore likely to be more useful in estimating biomass in mycelial bioprocesses. Furthermore, the NIR reflectance spectra of dried biomass were found to show correlations to the morphological variations introduced, suggesting that NIR spectra may be useful in obtaining morphology related information. © 2003 Wiley Periodicals, Inc. Biotechnol Bioeng 82: 715-724, 2003. en_US
dc.format.extent 10 en_US
dc.relation.ispartof Biotechnology and Bioengineering en_US
dc.subject near-infrared en_US
dc.subject morphology en_US
dc.subject mycelial en_US
dc.subject Streptomyces en_US
dc.subject bioprocess en_US
dc.title Influence of morphology on the near-infrared spectra of mycelial biomass and its implications in bioprocess monitoring en_US
dc.contributor.pbl Aberystwyth University en_US
dc.contributor.pbl Institute of Biological, Environmental and Rural Sciences en_US

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