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dc.contributor.author Gerard J. en_US
dc.contributor.author T. en_US
dc.contributor.author S. en_US
dc.contributor.author Takahito en_US
dc.contributor.author T. en_US
dc.contributor.author Yuji en_US
dc.date.accessioned 2009-11-02T16:36:02Z
dc.date.available 2009-11-02T16:36:02Z
dc.date.issued 2001-05 en_US
dc.identifier http://dx.doi.org/10.1016/S0031-9422(00)00440-4 en_US
dc.identifier.citation Bishop , G J , Yokota , T , Takatsuto , S , Nomura , T , Sato , T & Kamiya , Y 2001 , ' Accumulation of 6-deoxocathasterone and 6-deoxocastasterone in Arabidopsis, pea and tomato is suggestive of common rate-limiting steps in brassinosteroid biosynthesis ' Phytochemistry , vol 57 , no. 2 , pp. 171-178 . , 10.1016/S0031-9422(00)00440-4 en_US
dc.identifier.other PURE: 126635 en_US
dc.identifier.other dspace: 2160/3387 en_US
dc.identifier.uri http://hdl.handle.net/2160/3387
dc.description.abstract To gain a better understanding of brassinosteroid biosynthesis, the levels of brassinosteroids and sterols related to brassinolide biosynthesis in Arabidopsis, pea, and tomato plants were quantified by gas chromatography-selected ion monitoring. In these plants, the late C-6 oxidation pathway was found to be the predominant pathway in the synthesis of castasterone. Furthermore, all these plant species had similar BR profiles, suggesting the presence of common biosynthetic control mechanisms. The especially high levels of 6-deoxocathasterone and 6-deoxocastasterone may indicate that their respective conversions to 6-deoxoteasterone and castasterone are regulated in planta and hence are important rate-limiting steps in brassinosteroid biosynthesis. Other possible rate-limiting reactions, including the conversion of campestanol to 6-deoxocathasteonre, are also discussed. Tomato differs from Arabidopsis and pea in that tomato contains 28-norcastasterone as a biologically active brassinosteroid, and that its putative precursors, cholesterol and its relatives are the major sterols. en_US
dc.format.extent 8 en_US
dc.relation.ispartof Phytochemistry en_US
dc.subject Arabidopsis thaliana en_US
dc.subject Cruciferae en_US
dc.subject Pisum sativum en_US
dc.subject Leguminosae en_US
dc.subject Garden pea en_US
dc.subject Lycopersicon esculentum en_US
dc.subject Solanaccae en_US
dc.subject Tomato en_US
dc.subject Brassinosteroid en_US
dc.subject Biosynthesis en_US
dc.title Accumulation of 6-deoxocathasterone and 6-deoxocastasterone in Arabidopsis, pea and tomato is suggestive of common rate-limiting steps in brassinosteroid biosynthesis en_US
dc.contributor.pbl Aberystwyth University en_US
dc.contributor.pbl Institute of Biological, Environmental and Rural Sciences en_US


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