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dc.contributor.author Bishop, Gerard J.
dc.contributor.author Yokota, T.
dc.contributor.author Takatsuto, S.
dc.contributor.author Nomura, Takahito
dc.contributor.author Sato, T.
dc.contributor.author Kamiya, Yuji
dc.date.accessioned 2009-11-02T16:36:02Z
dc.date.available 2009-11-02T16:36:02Z
dc.date.issued 2001-05
dc.identifier.citation Bishop , G J , Yokota , T , Takatsuto , S , Nomura , T , Sato , T & Kamiya , Y 2001 , ' Accumulation of 6-deoxocathasterone and 6-deoxocastasterone in Arabidopsis, pea and tomato is suggestive of common rate-limiting steps in brassinosteroid biosynthesis ' Phytochemistry , vol 57 , no. 2 , pp. 171-178 . , 10.1016/S0031-9422(00)00440-4 en
dc.identifier.issn 0031-9422
dc.identifier.other PURE: 126635
dc.identifier.other dspace: 2160/3387
dc.identifier.uri http://hdl.handle.net/2160/3387
dc.description Nomura, T., Sato, T., Bishop, G. J., Kamiya, Y., Takatsuto, S., Yokota, T. (2001). Accumulation of 6-deoxocathasterone and 6-deoxocastasterone in Arabidopsis, pea and tomato is suggestive of common rate-limiting steps in brassinosteroid biosynthesis. Phytochemistry, 57, (2), 171-178. Sponsorship: Grand-in-Aid for Scientific Research from the Ministry of Education, Science, Sports and Culture of Japan/British Council/Royal Society en
dc.description.abstract To gain a better understanding of brassinosteroid biosynthesis, the levels of brassinosteroids and sterols related to brassinolide biosynthesis in Arabidopsis, pea, and tomato plants were quantified by gas chromatography-selected ion monitoring. In these plants, the late C-6 oxidation pathway was found to be the predominant pathway in the synthesis of castasterone. Furthermore, all these plant species had similar BR profiles, suggesting the presence of common biosynthetic control mechanisms. The especially high levels of 6-deoxocathasterone and 6-deoxocastasterone may indicate that their respective conversions to 6-deoxoteasterone and castasterone are regulated in planta and hence are important rate-limiting steps in brassinosteroid biosynthesis. Other possible rate-limiting reactions, including the conversion of campestanol to 6-deoxocathasteonre, are also discussed. Tomato differs from Arabidopsis and pea in that tomato contains 28-norcastasterone as a biologically active brassinosteroid, and that its putative precursors, cholesterol and its relatives are the major sterols. en
dc.format.extent 8 en
dc.language.iso eng
dc.relation.ispartof Phytochemistry en
dc.subject Arabidopsis thaliana en
dc.subject Cruciferae en
dc.subject Pisum sativum en
dc.subject Leguminosae en
dc.subject Garden pea en
dc.subject Lycopersicon esculentum en
dc.subject Solanaccae en
dc.subject Tomato en
dc.subject Brassinosteroid en
dc.subject Biosynthesis en
dc.title Accumulation of 6-deoxocathasterone and 6-deoxocastasterone in Arabidopsis, pea and tomato is suggestive of common rate-limiting steps in brassinosteroid biosynthesis en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1016/S0031-9422(00)00440-4
dc.contributor.institution Aberystwyth University en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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