Show simple item record Cresswell, A. Sackville Hamilton, N. R. Roy, A. K. Viegas, B. M. F. 2009-11-09T15:23:59Z 2009-11-09T15:23:59Z 2001-12-21
dc.identifier.citation Cresswell , A , Sackville Hamilton , N R , Roy , A K & Viegas , B M F 2001 , ' Use of amplified fragment length polymorphism markers to assess genetic diversity of Lolium species from Portugal ' Molecular Ecology , vol 10 , no. 1 , pp. 229-241 . DOI: 10.1046/j.1365-294X.2001.01170.x en
dc.identifier.issn 0962-1083
dc.identifier.other PURE: 128028
dc.identifier.other PURE UUID: f6d227b9-be43-47bf-a74d-755da27f45ff
dc.identifier.other dspace: 2160/3454
dc.identifier.other DSpace_20121128.csv: row: 2702
dc.identifier.other Scopus: 0035146132
dc.description Cresswell, A., Sackville Hamilton, N. R., Roy, A. K., Viegas, B. M. F. (2001). Use of AFLP markers to assess genetic diversity of Lolium species from Portugal. Molecular Ecology, 10, (1), 229-241. en
dc.description.abstract We evaluated the use of amplified fragment length polymorphism (AFLP) markers to distinguish genotypes, populations and species of Lolium. Accessions of two species Lolium perenne and Lolium multiflorum and their hybrid Lolium × hybridum, collected by the Institute of Grass and Environmental Research in 1995 from locations across Portugal, were used. The genetic variation within and between populations from the extremes of latitude and altitude was determined and assessed. Three primer pair combinations generated 765 polymorphic bands. Principal coordinate analysis of similarities between 127 plants showed high dimensionality in the data. Axes 1–3 were associated primarily with species differences, axes 4–14 with population differences within species and axis 15 onwards with within population differences. UPGMA analysis confirmed the groupings. The three populations of L. perenne formed a discrete cluster widely separated from all other populations. There were two distinct groups of L. × hybridum, of which one was similar to and overlapped with L.multiflorum and the second formed a distinct cluster. Analyses of individual bands showed that every inter- and intraspecific contrast involved a different sets of bands, again confirming the high dimensionality of the data. No single band was strictly diagnostic of any population or species. Nevertheless, the UPGMA analysis showed little or no overlap between populations. Thus, despite the high ratio of within-to-between population genetic variance, the full AFLP banding pattern of each genotype is a relatively reliable fingerprint diagnostic of its parent population. The high dimensionality implies that many different factors contribute to the differences observed. This adds to the potential value of the methodology, since it implies that there is a reasonably high likelihood of finding bands relevant to a given environmental gradient or other factor influencing the distribution of genetic diversity. en
dc.format.extent 13 en
dc.language.iso eng
dc.relation.ispartof Molecular Ecology en
dc.rights en
dc.title Use of amplified fragment length polymorphism markers to assess genetic diversity of Lolium species from Portugal en
dc.type /dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en

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