Gene structure of the extracellular glutathione S-transferase from Onchocerca volvulus and its overexpression and promoter analysis in transgenic Caenorhabditis elegans

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dc.contributor.author Brophy, Peter M.
dc.contributor.author Krause, S.
dc.contributor.author Walter, R. D.
dc.contributor.author Liebau, E.
dc.contributor.author Fischer, P.
dc.contributor.author Sommer, A.
dc.date.accessioned 2009-11-09T15:37:32Z
dc.date.available 2009-11-09T15:37:32Z
dc.date.issued 2001-10-01
dc.identifier.citation Brophy , P M , Krause , S , Walter , R D , Liebau , E , Fischer , P & Sommer , A 2001 , ' Gene structure of the extracellular glutathione S-transferase from Onchocerca volvulus and its overexpression and promoter analysis in transgenic Caenorhabditis elegans ' Molecular and Biochemical Parasitology , pp. 145-154 . , 10.1016/S0166-6851(01)00342-5 en
dc.identifier.other PURE: 128514
dc.identifier.other dspace: 2160/3456
dc.identifier.uri http://hdl.handle.net/2160/3456
dc.description Krause, S., Sommer, A., Fischer, P., Brophy, P. M., Walter, R. D., Liebau, E. (2001). Gene structure of the extracellular glutathione S-transferase from Onchocerca volvulus and its overexpression and promoter analysis in transgenic Caenorhabditis elegans. Molecular and Biochemical Parasitology, 117, (2), 145-154. Sponsorship: Deutsche Forschungsgemeinschaft (DFG-projects Li791/1-4). en
dc.description.abstract Two highly similar genes encoding unique extracellular, glycosylated glutathione S-transferases (GSTs) of the human-pathogenic nematode, Onchocerca volvulus (Ov-GST1a and Ov-GST1b), have been isolated and characterised. The genes are ≈3 kb in length and consist of seven exons interrupted by introns of ≈100 bp in length, with the exception of intron II, which is ≈1.6 kb in length. Interestingly, exon I and II encode a signal peptide and an N-terminal extension before sequence homology to other GSTs begins. The 5′ flanking region was sequenced and analysed for transcription factor binding sites. Consistent with the lack of a TATA box, analysis of the mRNAs by primer extension showed multiple transcription start sites spread over a 60 bp region. To examine the activity and specificity of the Ov-GST1a gene promoter, we have exploited Caenorhabditis elegans as a heterologous transformation system. To analyse whether transgenic C. elegans are able to carry out processing and post-transcriptional modifications of the Ov-GST1a correctly, the protein was ectopically overexpressed in C. elegans. The parasite-derived Ov-GST1a gene product was correctly processed in transgenic C. elegans and posttranslational modifications, such as signal peptide cleavage and N-glycosylation, were performed successfully. This further demonstrates the potential of C. elegans as a host for expression of candidate vaccine antigens from O. volvulus and affirms the role of C. elegans as a model for parasitic nematodes. en
dc.format.extent 10 en
dc.language.iso eng
dc.relation.ispartof Molecular and Biochemical Parasitology en
dc.title Gene structure of the extracellular glutathione S-transferase from Onchocerca volvulus and its overexpression and promoter analysis in transgenic Caenorhabditis elegans en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1016/S0166-6851(01)00342-5
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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