Visualization and partial characterization of the ECM of Pestalotia malicola on artificial and natural substrata

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dc.contributor.author Nicholson, R. L.
dc.contributor.author Thomas, Barry J.
dc.contributor.author Carver, Timothy L. W.
dc.contributor.author Gevens, A. J.
dc.date.accessioned 2009-11-10T09:21:25Z
dc.date.available 2009-11-10T09:21:25Z
dc.date.issued 2001-06
dc.identifier.citation Nicholson , R L , Thomas , B J , Carver , T L W & Gevens , A J 2001 , ' Visualization and partial characterization of the ECM of Pestalotia malicola on artificial and natural substrata ' Physiological and Molecular Plant Pathology , vol 58 , no. 6 , pp. 277-285 . , 10.1006/pmpp.2001.0338 en
dc.identifier.issn 0885-5765
dc.identifier.other PURE: 127999
dc.identifier.other dspace: 2160/3466
dc.identifier.uri http://hdl.handle.net/2160/3466
dc.description Gevens, A. J., Carver, T. L. W., Thomas, B. J., Nicholson, R. L. (2001). Visualization and partial characterization of the ECM of Pestalotia malicola on artificial and natural substrata.  Visualization and partial characterization of the ECM of Pestalotia malicola on artificial and natural substrata, 277-285 en
dc.description.abstract The extracellular matrix (ECM) released by conidia of Pestalotia malicola when incubated on artificial and natural substrata was investigated. Scanning electron microscopy (SEM) images indicated the presence of ECM beneath conidial appendages. Stains for non-specific esterase activity revealed that enzyme activity was initially present at the conidial appendages. Gelatin peel tests observed with light microscopy and SEM indicated that a strong initial spore adhesion occurs at the appendages flanking the conidium body. That the ECM was seen beneath firmly adhered conidial appendages suggests that it has a role in adhesion. Harvested ECM material from germinating conidia was deglycosylated and acetylated for GC/MS monosaccharide identification. Mole percent calculations show mannose to be the predominant monosaccharide present. en
dc.format.extent 9 en
dc.language.iso eng
dc.relation.ispartof Physiological and Molecular Plant Pathology en
dc.title Visualization and partial characterization of the ECM of Pestalotia malicola on artificial and natural substrata en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1006/pmpp.2001.0338
dc.contributor.institution Aberystwyth University en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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