Developmental expression of activin/inhibin alpha- and beta(A)-subunit genes in the gonads of male and female chick embryos

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dc.contributor.author Onagbesan, O. M.
dc.contributor.author Bruggeman, V.
dc.contributor.author Volckaert, G.
dc.contributor.author Vanmontfort, D.
dc.contributor.author Decuypere, E.
dc.contributor.author Safi, M.
dc.date.accessioned 2009-11-10T11:24:54Z
dc.date.available 2009-11-10T11:24:54Z
dc.date.issued 2001-06
dc.identifier.citation Onagbesan , O M , Bruggeman , V , Volckaert , G , Vanmontfort , D , Decuypere , E & Safi , M 2001 , ' Developmental expression of activin/inhibin alpha- and beta(A)-subunit genes in the gonads of male and female chick embryos ' General and Comparative Endocrinology , pp. 304-311 . en
dc.identifier.other PURE: 128101
dc.identifier.other dspace: 2160/3472
dc.identifier.uri http://hdl.handle.net/2160/3472
dc.description Safi, M., Onagbesan, O. M., Volckaert, G., Vanmontfort, D., Bruggeman, V., Decuypere, E (2001). Developmental expression of activin/inhibin alpha- and beta(A)-subunit genes in the gonads of male and female chick embryos. General and Comparative Endocrinology, 122, (3), 304-311. Sponsorship: Grant for foreign advanced doctoral researchers of the Catholic University of Leuven. en
dc.description.abstract The expression of inhibin α- and βA-subunits was investigated in gonads of male and female chick embryos during the last week of their 21-day incubation period. Fertilized Hisex brown laying hen eggs were incubated at 37.8 ± 0.2° and 60% relative humidity in an automatic forced-draft incubator with constant lighting. Embryos were killed after 14, 18, and 21 days of incubation, sexed by macroscopical inspection of the gonadal phenotype, and further dissected to obtain the gonads. Total RNA was isolated using the ultraspec RNA method. The expression of α- and βA-subunits was evaluated by competitive RT-PCR. Significant differences were found within and between sexes in the expression of the α- and βA-subunits. The level of the α-subunit in the testis was about 23-fold higher than that in the ovary at all ages. Testicular content of inhibin α mRNA levels was similar at days 14 and 18 but declined significantly at day 21 of incubation, whereas no significant differences were observed between the three age groups in the ovary. Testicular and ovarian inhibin βA-subunit increased significantly from day 14 to day 18 followed by a significant decline before hatch. However, inhibin βA level at day 14 was significantly higher in the ovary than in the testis. At days 18 and 21, there were no differences in the levels of the inhibin βA in the sexes. The expression of inhibin βA-subunit in the ovary was significantly higher than that of the α-subunit at all ages. In the testis, however, the expression of the βA-subunit was higher at days 18 and 21 than at day 14. The sex difference in gonadal inhibin subunits expression suggests differential roles of inhibin/activin in the development of the chicken gonads. The changing level of expression during incubation also suggests changing biological roles within sexes. en
dc.format.extent 8 en
dc.language.iso eng
dc.relation.ispartof General and Comparative Endocrinology en
dc.title Developmental expression of activin/inhibin alpha- and beta(A)-subunit genes in the gonads of male and female chick embryos en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1006/gcen.2000.7546
dc.contributor.institution Aberystwyth University en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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