Plant sterol 14 alpha-demethylase affinity for azole fungicides

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dc.contributor.author Lamb, David C.
dc.contributor.author Kelly, Diane E.
dc.contributor.author Bak, Soren
dc.contributor.author Warrilow, Andrew G. S.
dc.contributor.author Kelly, Steven L.
dc.contributor.author Cannieux, Michel
dc.contributor.author Kahn, Rachel A.
dc.contributor.author Manning, Nigel J.
dc.date.accessioned 2009-11-11T13:22:07Z
dc.date.available 2009-11-11T13:22:07Z
dc.date.issued 2001-06-15
dc.identifier.citation Lamb , D C , Kelly , D E , Bak , S , Warrilow , A G S , Kelly , S L , Cannieux , M , Kahn , R A & Manning , N J 2001 , ' Plant sterol 14 alpha-demethylase affinity for azole fungicides ' Biochemical and Biophysical Research Communications , vol 284 , no. 3 , pp. 845-849 . , 10.1006/bbrc.2001.5010 en
dc.identifier.issn 1090-2104
dc.identifier.other PURE: 128487
dc.identifier.other dspace: 2160/3508
dc.identifier.uri http://hdl.handle.net/2160/3508
dc.description Lamb, D. C., Cannieux, M., Warrilow, A. G. S., Bak, S., Kahn, R. A., Manning, N. J., Kelly, D. E., Kelly, S. L. (2001). Plant sterol 14 alpha-demethylase affinity for azole fungicides.  Biochemical and Biophysical Research Communications, 284, (3), 845-849. Sponsorship: Biotechnology and Biological Science Research Council (BBSRC) en
dc.description.abstract Azole fungicides were thought to have much greater affinity for the fungal cytochrome P450 enzyme, sterol 14α-demthylase (CYP51) than the plant orthologue. Using purified CYP51 from the plant Sorghum bicolor L Moenech, a direct comparison of the sensitivity to the fungicides triadimenol and tebuconazole has been carried out. S. bicolor CYP51 was purified to homogenity as determined by SDS–PAGE and specific heme content. Addition of the azole fungicides triadimenol and tebuconazole induced type II spectral changes, with saturation occurring at equimolar azole/P450 concentrations. Inhibition of reconstituted activities revealed only a threefold insensitivity of the plant CYP51 compared to a fungal CYP51, from the phytopathogen Ustilago maydis, as judged by IC50 values. The implications for fungicide mode of action and application are discussed. en
dc.format.extent 5 en
dc.language.iso eng
dc.relation.ispartof Biochemical and Biophysical Research Communications en
dc.title Plant sterol 14 alpha-demethylase affinity for azole fungicides en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1006/bbrc.2001.5010
dc.contributor.institution Aberystwyth University en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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