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dc.contributor.author David C. en_US
dc.contributor.author Diane E. en_US
dc.contributor.author Soren en_US
dc.contributor.author Andrew G. S. en_US
dc.contributor.author Steven L. en_US
dc.contributor.author Michel en_US
dc.contributor.author Rachel A. en_US
dc.contributor.author Nigel J. en_US
dc.date.accessioned 2009-11-11T13:22:07Z
dc.date.available 2009-11-11T13:22:07Z
dc.date.issued 2001-06-15 en_US
dc.identifier http://dx.doi.org/10.1006/bbrc.2001.5010 en_US
dc.identifier.citation Lamb , D C , Kelly , D E , Bak , S , Warrilow , A G S , Kelly , S L , Cannieux , M , Kahn , R A & Manning , N J 2001 , ' Plant sterol 14 alpha-demethylase affinity for azole fungicides ' Biochemical and Biophysical Research Communications , vol 284 , no. 3 , pp. 845-849 . , 10.1006/bbrc.2001.5010 en_US
dc.identifier.other PURE: 128487 en_US
dc.identifier.other dspace: 2160/3508 en_US
dc.identifier.uri http://hdl.handle.net/2160/3508
dc.description.abstract Azole fungicides were thought to have much greater affinity for the fungal cytochrome P450 enzyme, sterol 14α-demthylase (CYP51) than the plant orthologue. Using purified CYP51 from the plant Sorghum bicolor L Moenech, a direct comparison of the sensitivity to the fungicides triadimenol and tebuconazole has been carried out. S. bicolor CYP51 was purified to homogenity as determined by SDS–PAGE and specific heme content. Addition of the azole fungicides triadimenol and tebuconazole induced type II spectral changes, with saturation occurring at equimolar azole/P450 concentrations. Inhibition of reconstituted activities revealed only a threefold insensitivity of the plant CYP51 compared to a fungal CYP51, from the phytopathogen Ustilago maydis, as judged by IC50 values. The implications for fungicide mode of action and application are discussed. en_US
dc.format.extent 5 en_US
dc.relation.ispartof Biochemical and Biophysical Research Communications en_US
dc.title Plant sterol 14 alpha-demethylase affinity for azole fungicides en_US
dc.contributor.pbl Aberystwyth University en_US
dc.contributor.pbl Institute of Biological, Environmental and Rural Sciences en_US


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