Brassinosteroid-6-oxidases from Arabidopsis and tomato catalyze multiple C-6 oxidations in brassinosteroid biosynthesis

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dc.contributor.author Fujioka, Shozo
dc.contributor.author Yokota, Takao
dc.contributor.author Yoshida, Shigeo
dc.contributor.author Shimada, Yukihisa
dc.contributor.author Kamiya, Yuji
dc.contributor.author Takatsuto, Suguru
dc.contributor.author Kushiro, Masayo
dc.contributor.author Miyauchi, Narumasa
dc.contributor.author Nomura, Takahito
dc.contributor.author Bishop, Gerard J.
dc.date.accessioned 2009-11-12T09:42:55Z
dc.date.available 2009-11-12T09:42:55Z
dc.date.issued 2001-06
dc.identifier.citation Fujioka , S , Yokota , T , Yoshida , S , Shimada , Y , Kamiya , Y , Takatsuto , S , Kushiro , M , Miyauchi , N , Nomura , T & Bishop , G J 2001 , ' Brassinosteroid-6-oxidases from Arabidopsis and tomato catalyze multiple C-6 oxidations in brassinosteroid biosynthesis ' Plant Physiology , vol 126 , no. 2 , pp. 770-770 . en
dc.identifier.issn 0032-0889
dc.identifier.other PURE: 128137
dc.identifier.other dspace: 2160/3509
dc.identifier.uri http://hdl.handle.net/2160/3509
dc.identifier.uri http://www.plantphysiol.org/cgi/content/full/126/2/770 en
dc.description Shimada, Y., Fujioka, S., Miyauchi, N., Kushiro, M., Takatsuto, S., Nomura, T., Yokota, T., Kamiya, Y., Bishop, G. J., Yoshida, S. (2001). Brassinosteroid-6-oxidases from Arabidopsis and tomato catalyze multiple C-6 oxidations in brassinosteroid biosynthesis.   Plant Physiology, 126, (2), 770-770. en
dc.description.abstract Brassinosteroids (BRs) are steroidal plant hormones that are essential for growth and development. It has been proposed that BRs are synthesized via two parallel pathways, the early and late C-6 oxidation pathways according to the C-6 oxidation status. The tomato (Lycopersicon esculentum) Dwarf gene encodes a cytochrome P450 that has been shown to catalyze the C-6 oxidation of 6-deoxocastasterone to castasterone. We isolated an Arabidopsis ortholog (AtBR6ox gene) of the tomato Dwarf gene. The encoded polypeptide has characteristics of P450s and is classified into the CYP85 family. The AtBR6ox and tomato Dwarf gene were expressed in yeast and the ability of the transformed yeast cells to metabolize 6-deoxo-BRs was tested. Metabolites were analyzed by gas chromatography-mass spectrometry. Both enzymes catalyze multiple steps in BR biosynthesis: 6-deoxoteasterone to teasterone, 3-dehydro-6-deoxoteasterone to 3-dehydroteasterone, 6-deoxotyphasterol to typhasterol, and 6-deoxocastasterone to castasterone. Our results indicate that the AtBR6ox gene and the tomato Dwarf gene encode steroid-6-oxidases and that these enzymes have a broad substrate specificity. This suggests that the BR biosynthetic pathway consists of a metabolic grid rather than two separate parallel pathways. en
dc.format.extent 1 en
dc.language.iso eng
dc.relation.ispartof Plant Physiology en
dc.title Brassinosteroid-6-oxidases from Arabidopsis and tomato catalyze multiple C-6 oxidations in brassinosteroid biosynthesis en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/​10.​1104/​pp.​126.​2.​770
dc.contributor.institution Aberystwyth University en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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