Export of cytochrome P450 105D1 to the periplasmic space of Escherichia coli

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dc.contributor.author Ugochukwu, C. C.
dc.contributor.author Lamb, David C.
dc.contributor.author Kaderbhai, Mustak A.
dc.contributor.author Kelly, Steven L.
dc.date.accessioned 2009-11-13T09:16:32Z
dc.date.available 2009-11-13T09:16:32Z
dc.date.issued 2001
dc.identifier.citation Ugochukwu , C C , Lamb , D C , Kaderbhai , M A & Kelly , S L 2001 , ' Export of cytochrome P450 105D1 to the periplasmic space of Escherichia coli ' Applied and Environmental Microbiology , pp. 2136-2138 . , 10.1128/AEM.67.5.2136-2138.2001 en
dc.identifier.other PURE: 128443
dc.identifier.other dspace: 2160/3518
dc.identifier.uri http://hdl.handle.net/2160/3518
dc.description Kaderbhai, M. A., Ugochukwu, C. C., Kelly, S. L., Lamb, D. C. (2001). Export of cytochrome P450 105D1 to the periplasmic space of Escherichia coli. Applied and Environmental Microbiology, 67, (5), 2136-2138 Sponsorship: BBSRC en
dc.description.abstract CYP105D1, a cytochrome P450 from Streptomyces griseus, was appended at its amino terminus to the secretory signal of Escherichia coli alkaline phosphatase and placed under the transcriptional control of the native phoA promoter. Heterologous expression in E. coli phosphate-limited medium resulted in abundant synthesis of recombinant CYP105D1 that was translocated across the bacterial inner membrane and processed to yield authentic, heme-incorporated P450 within the periplasmic space. Cell extract and whole-cell activity studies showed that the periplasmically located CYP105D1 competently catalyzed NADH-dependent oxidation of the xenobiotic compounds benzo[a]pyrene and erythromycin, further revealing the presence in the E. coli periplasm of endogenous functional redox partners. This system offers substantial advantages for the application of P450 enzymes to whole-cell biotransformation strategies, where the ability of cells to take up substrates or discard products may be limited. en
dc.format.extent 3 en
dc.language.iso eng
dc.relation.ispartof Applied and Environmental Microbiology en
dc.title Export of cytochrome P450 105D1 to the periplasmic space of Escherichia coli en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1128/AEM.67.5.2136-2138.2001
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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