Production of immobilized penicillin acylase using aqueous polymer systems for enzyme purification and in situ immobilization

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dc.contributor.author Guan, Y. H.
dc.contributor.author Brook, A. H.
dc.contributor.author Lilley, T. H.
dc.date.accessioned 2009-11-20T09:34:24Z
dc.date.available 2009-11-20T09:34:24Z
dc.date.issued 2001-02-01
dc.identifier.citation Guan , Y H , Brook , A H & Lilley , T H 2001 , ' Production of immobilized penicillin acylase using aqueous polymer systems for enzyme purification and in situ immobilization ' Enzyme and Microbial Technology , vol 28 , no. 2-3 , pp. 2-3 . en
dc.identifier.issn 0141-0229
dc.identifier.other PURE: 129206
dc.identifier.other dspace: 2160/3564
dc.identifier.uri http://hdl.handle.net/2160/3564
dc.description Guan, Y. H., Lilley, T. H., Brook, A. H. (2001). Production of immobilized penicillin acylase using aqueous polymer systems for enzyme purification and in situ immobilization.  Enzyme and Microbial Technology, 28, (2-3), 218-224. Sponsorship: SmithKline Beecham Pharmaceuticals (Worthing) and the University of Wales Aberystwyth en
dc.description.abstract A novel approach for the isolation and purification of penicillin acylase (PA), which couples aqueous two-phase partitioning and enzyme immobilization has been investigated. A PA yield of 90% was achieved by treating E. coli cells with 4% butyl acetate, freeze-thawing step, and pressure homogenization. PA purification (93% recovery) was achieved by (1) removing cell debris via precipitation with polyethylene glycol (PEG 2000); (2) aqueous two-phase partitioning using a PEG 2000 + phosphate system (87% recovery). An in situ enzyme immobilization approach, using oxirane acrylic or aldehyde-agarose beads dispersed in the PEG-rich phase, was explored for the conversion of penicillin G to 6-aminopenicillanic acid. An appropriate immobilization reaction time was found. The catalytic performance of the enzyme, when immobilized, was found not to be affected by recycling of the phase-forming components. en
dc.format.extent 2 en
dc.language.iso eng
dc.relation.ispartof Enzyme and Microbial Technology en
dc.title Production of immobilized penicillin acylase using aqueous polymer systems for enzyme purification and in situ immobilization en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1016/S0141-0229(00)00305-7
dc.contributor.institution Aberystwyth University en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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