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dc.contributor.author Harvey, Linda M.
dc.contributor.author McNeil, B.
dc.contributor.author Vaidyanathan, S.
dc.contributor.author Mohan, P.
dc.contributor.author Arnold, S. A.
dc.contributor.author Matheson, L.
dc.date.accessioned 2009-11-24T17:15:27Z
dc.date.available 2009-11-24T17:15:27Z
dc.date.issued 2001-09-05
dc.identifier.citation Harvey , L M , McNeil , B , Vaidyanathan , S , Mohan , P , Arnold , S A & Matheson , L 2001 , ' Assessment of near-infrared spectral information for rapid monitoring of bioprocess quality ' Biotechnology and Bioengineering , vol 74 , no. 5 , pp. 376-388 . , 10.1002/bit.1128 en
dc.identifier.issn 1097-0290
dc.identifier.other PURE: 129946
dc.identifier.other dspace: 2160/3589
dc.identifier.uri http://hdl.handle.net/2160/3589
dc.description Vaidyanathan, S., Arnold, S. A., Matheson, L., Mohan, P., McNeil, B., Harvey, L. M. (2001). Assessment of near-infrared spectral information for rapid monitoring of bioprocess quality.  Biotechnology and Bioengineering, 74, (5), 376-388. en
dc.description.abstract Access to real-time process information is desirable for consistent and efficient operation of bioprocesses. Near-infrared spectroscopy (NIRS) is known to have potential for providing real-time information on the quantitative levels of important bioprocess variables. However, given the fact that a typical NIR spectrum encompasses information regarding almost all the constituents of the sample matrix, there are few case studies that have investigated the spectral details for applications in bioprocess quality assessment or qualitative bioprocess monitoring. Such information would be invaluable in providing operator-level assistance on the progress of a bioprocess in industrial-scale productions. We investigated this aspect and report the results of our investigation. Near-infrared spectral information derived from scanning unprocessed culture fluid (broth) samples from a complex antibiotic production process was assessed for a data set that incorporated bioprocess variations. Principal component analysis was applied to the spectral data and the loadings and scores of the principal components studied. Changes in the spectral information that corresponded to variations in the bioprocess could be deciphered. Despite the complexity of the matrix, near-infrared spectra of the culture broth are shown to have valuable information that can be deconvoluted with the help of factor analysis techniques such as principal component analysis (PCA). Although complex to interpret, the loadings and score plots are shown to offer potential in process diagnosis that could be of value in the rapid assessment of process quality, and in data assessment prior to quantitative model development. en
dc.format.extent 13 en
dc.language.iso eng
dc.relation.ispartof Biotechnology and Bioengineering en
dc.subject Streptomyces fradiae en
dc.subject antibiotic en
dc.subject fermentation en
dc.subject spectroscopy en
dc.subject multivariate analysis en
dc.title Assessment of near-infrared spectral information for rapid monitoring of bioprocess quality en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1002/bit.1128
dc.contributor.institution Aberystwyth University en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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