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dc.contributor.author Kell, Douglas B.
dc.contributor.author Goodacre, Royston
dc.contributor.author Rowland, Jeremy John
dc.contributor.author Vaidyanathan, S.
dc.date.accessioned 2009-11-25T11:52:49Z
dc.date.available 2009-11-25T11:52:49Z
dc.date.issued 2001-08-02
dc.identifier.citation Kell , D B , Goodacre , R , Rowland , J J & Vaidyanathan , S 2001 , ' Discrimination of aerobic endospore-forming bacteria via electrospray-ionization mass spectrometry of whole cell suspensions ' Analytical Chemistry , pp. 4134-4144 . , 10.1021/ac0103524 en
dc.identifier.issn 1520-6882
dc.identifier.other PURE: 129405
dc.identifier.other dspace: 2160/3605
dc.identifier.uri http://hdl.handle.net/2160/3605
dc.description Vaidyanathan, S., Rowland, J. J., Kell, D. B., Goodacre, R. (2001). Discrimination of aerobic endospore-forming bacteria via electrospray-ionization mass spectrometry of whole cell suspensions. Analytical Chemistry, 73, (17), 4134-4144. Sponsorship: BBSRC en
dc.description.abstract Direct injection electrospray ionization mass spectrometry (ESI-MS) without prior analyte separation was investigated for the analysis of whole cell suspensions of bacteria. Thirty-six strains of aerobic endospore-forming bacteria, consisting of six Bacillus species and one Brevibacillus species, were studied. ESI was performed in the positive ion mode on the bacterial suspensions. Several peaks in the range of 250−1500 m/z were observed to contribute to variations in the spectral information among the species. Application of cluster analysis to the spectral data showed that this ESI-MS technique was capable of discriminating strains of the species B. subtilis. This investigation demonstrates the feasibility of measuring liquid samples with minimal sample preparation that can be useful for discrimination at the subspecies level. A change in the cone potential in the electrospray ion source was found to influence the spectral information of representative strains of all of the seven species tested. This has implications with respect to optimizing the experimental conditions for discriminatory purposes, but could offer additional information with respect to microbial characterization. A comparison of the spectra of whole cell suspensions and cell-free supernatants showed a high degree of similarity between the two, which has consequences for ease of automation. en
dc.format.extent 11 en
dc.language.iso eng
dc.relation.ispartof Analytical Chemistry en
dc.title Discrimination of aerobic endospore-forming bacteria via electrospray-ionization mass spectrometry of whole cell suspensions en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1021/ac0103524
dc.contributor.institution Department of Computer Science en
dc.description.status Peer reviewed en


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