Expression studies of superoxide dismutases in nodules and leaves of transgenic alfalfa reveal abundance of iron-containing isozymes, post-translational regulation and compensation of isoenzyme activities

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dc.contributor.author Arrese-Igor, Cesar
dc.contributor.author Ramos, J.
dc.contributor.author Minchin, Frank R.
dc.contributor.author Gonzalez, Esther M.
dc.contributor.author Becana, Manuel
dc.contributor.author Rubio, M. C.
dc.contributor.author Webb, K. Judith
dc.date.accessioned 2009-11-25T12:22:07Z
dc.date.available 2009-11-25T12:22:07Z
dc.date.issued 2001
dc.identifier.citation Arrese-Igor , C , Ramos , J , Minchin , F R , Gonzalez , E M , Becana , M , Rubio , M C & Webb , K J 2001 , ' Expression studies of superoxide dismutases in nodules and leaves of transgenic alfalfa reveal abundance of iron-containing isozymes, post-translational regulation and compensation of isoenzyme activities ' Molecular Plant-Microbe Interactions , pp. 1178-1188 . , 10.1094/MPMI.2001.14.10.1178 en
dc.identifier.issn 0894-0282
dc.identifier.other PURE: 130181
dc.identifier.other dspace: 2160/3609
dc.identifier.uri http://hdl.handle.net/2160/3609
dc.description Rubio, M. C., Ramos, J., Webb, K. J., Minchin, F. R., Gonzalez, E., Arrese-Igor, C., Becana, M. (2001). Expression studies of superoxide dismutases in nodules and leaves of transgenic alfalfa reveal abundance of iron-containing isozymes, post-translational regulation and compensation of isoenzyme activities. Molecular Plant-Microbe Interactions, 14, (10), 1178-1188. Sponsorship: Grant 2FD97-1101 from the Comisión Interministerial de Ciencia y Tecnología and the European Commission/grant PB98-0522 from the Dirección General de Investigación Científica (Spain)/ BBSRC. en
dc.description.abstract The composition of antioxidant enzymes, especially superoxide dismutase (SOD), was studied in one nontransgenic and three transgenic lines of nodulated alfalfa plants. Transgenic lines overproduced MnSOD in the mitochondria of nodules and leaves (line 1-10), MnSOD in the chloroplasts (line 4-6), and FeSOD in the chloroplasts (line 10-7). In nodules of line 10-7, the absence of transgeneencoded FeSOD activity was due to a lack of mRNA, whereas in nodules of line 4-6 the absence of transgeneencoded MnSOD activity was due to enzyme inactivation or degradation. Transgenic alfalfa showed a novel compensatory effect in the activities of MnSOD (mitochondrial) and FeSOD (plastidic) in the leaves, which was not caused by changes in the mRNA levels. These findings imply that SOD activity in plant tissues and organelles is regulated, at least partially, at the posttranslational level. All four lines had low CuZnSOD activities and an abundant FeSOD isozyme, especially in nodules, indicating that FeSOD performs important antioxidant functions other than the scavenging of superoxide radicals generated in photosynthesis. This was confirmed by the detection of FeSOD cDNAs and proteins in nodules of other legumes such as cowpea, pea, and soybean. The cDNA encoding alfalfa nodule FeSOD was characterized and the deduced protein found to contain a plastid transit peptide. A comparison of sequences and other properties reveals that there are two types of FeSODs in nodules. en
dc.format.extent 11 en
dc.language.iso eng
dc.relation.ispartof Molecular Plant-Microbe Interactions en
dc.title Expression studies of superoxide dismutases in nodules and leaves of transgenic alfalfa reveal abundance of iron-containing isozymes, post-translational regulation and compensation of isoenzyme activities en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1094/MPMI.2001.14.10.1178
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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