Show simple item record Deevska, G. Timanova-Atanasova, A. Jordanova, R. Barrett, John Bankov, I. Radoslavov, G. 2009-11-25T16:04:26Z 2009-11-25T16:04:26Z 2004-09-24
dc.identifier.citation Deevska , G , Timanova-Atanasova , A , Jordanova , R , Barrett , J , Bankov , I & Radoslavov , G 2004 , ' A native 13-kDa fatty acid binding protein from the liver fluke Fasciola hepatica ' Biochimica et Biophysica Acta (BBA) - General Subjects , vol 1674 , no. 2 , pp. 200-204 . DOI: 10.1016/j.bbagen.2004.06.018 en
dc.identifier.other PURE: 122789
dc.identifier.other PURE UUID: f8de4e17-027a-4c2e-bdd9-2081ec5bb608
dc.identifier.other dspace: 2160/3631
dc.identifier.other DSpace_20121128.csv: row: 2486
dc.identifier.other Scopus: 12344279869
dc.description Timanova-Atanasova, A., Jordanova, R., Radoslavov, G., Deevska, G., Bankov, I., Barrett, J. (2004). A native 13-kDa fatty acid binding protein from the liver fluke Fasciola hepatica. Biochimica et Biophysica Acta-General Subjects, 1674, (2), 200-204. Sponsorship: Deutsche Forshungsgemeinschaft (DFG) en
dc.description.abstract A 13-kDa fatty acid binding protein (FABP) (Fh13) has been isolated from the cytosol of adult Fasciola hepatica and its physicochemical and binding characteristics determined. Fh13 appears to exist as a dimer in native solution. Binding of the fluorescent fatty acid analogue 11-((5-dimethyl aminonaphthalene-1-sulfonyl) amino) undecanoic acid (DAUDA) to Fh13 results in changes in the emission spectrum, which are reversed by oleic acid. The binding activity for DAUDA determined from titration experiments revealed a single binding site per monomeric unit with Kd of 1.5 μM. The displacement of DAUDA by competitive nonfluorescent ligands allowed Kd values for oleic (2.5 μM), retinoic (2.8 μM), palmitic (4.1 μM) and arachidonic acid (6.1 μM) to be calculated. Ten commonly used anthelmintics were evaluated for binding to Fh13, but only bithionol showed binding activity commensurate with those of the putative natural ligands (Kd 6.8 μM). en
dc.format.extent 5 en
dc.language.iso eng
dc.relation.ispartof Biochimica et Biophysica Acta (BBA) - General Subjects en
dc.rights en
dc.title A native 13-kDa fatty acid binding protein from the liver fluke Fasciola hepatica en
dc.type /dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en

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