Degeneration of solventogenic Clostridium strains monitored by Fourier transform infrared spectroscopy of bacterial cells

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dc.contributor.author Gapes, J. R.
dc.contributor.author Schuster, K. C.
dc.contributor.author Goodacre, Royston
dc.contributor.author Young, Michael
dc.date.accessioned 2009-11-26T09:43:41Z
dc.date.available 2009-11-26T09:43:41Z
dc.date.issued 2001-11
dc.identifier.citation Gapes , J R , Schuster , K C , Goodacre , R & Young , M 2001 , ' Degeneration of solventogenic Clostridium strains monitored by Fourier transform infrared spectroscopy of bacterial cells ' Journal of Industrial Microbiology & Biotechnology , vol 27 , no. 5 , pp. 314-321 . , 10.1038/sj.jim.7000146 en
dc.identifier.issn 1476-5535
dc.identifier.other PURE: 130651
dc.identifier.other dspace: 2160/3641
dc.identifier.uri http://hdl.handle.net/2160/3641
dc.description Schuster, K. C., Goodacre, R., Gapes, J. R., Young, M. (2001). Degeneration of solventogenic Clostridium strains monitored by Fourier transform infrared spectroscopy of bacterial cells. Journal of Industrial Microbiology & Biotechnology, 27, (5), 314-321. Sponsorship: BBSRC / European Commission (FAIR-CT96-1201) en
dc.description.abstract Strain degeneration in solventogenic clostridia is a known problem in the technical acetone–butanol fermentation bioprocess, especially in the continuous process mode. Clostridial strain degeneration was studied by Fourier transform infrared (FT-IR) spectroscopy of the bacterial cells. Degenerative variant formation in two strains, Clostridium beijerinckii NCIMB 8052 and Clostridium species AA332, was detected spectroscopically. Colonies on solid media were sampled, or assayed directly in situ by IR microscopy. It has previously been shown that the distinctive acidogenic and solventogenic physiological phases of Clostridium acetobutylicum in liquid medium can be discriminated by FT-IR spectroscopy. This was confirmed here for C. beijerinckii NCIMB 8052. The proportion of degenerate cells in a mixed population in liquid medium could be quantified, as the spectral features change in different ways during the normal growth cycle of wild type organisms and degenerate variants in batch culture. This opens a new perspective for physiology-based process monitoring and control, especially of the continuous acetone–butanol fermentation. en
dc.format.extent 8 en
dc.language.iso eng
dc.relation.ispartof Journal of Industrial Microbiology & Biotechnology en
dc.subject Acetone-butanol fermentation en
dc.subject Clostridium en
dc.subject solventogenic fermentation en
dc.subject strain degeneration en
dc.subject FT-IR spectroscopy en
dc.subject fermentation process monitoring en
dc.title Degeneration of solventogenic Clostridium strains monitored by Fourier transform infrared spectroscopy of bacterial cells en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1038/sj.jim.7000146
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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