Characterization of an autostimulatory substance produced by Escherichia coli

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dc.contributor.author Kell, Douglas B.
dc.contributor.author Weichart, D. H.
dc.date.accessioned 2009-11-26T09:46:11Z
dc.date.available 2009-11-26T09:46:11Z
dc.date.issued 2001-07
dc.identifier.citation Kell , D B & Weichart , D H 2001 , ' Characterization of an autostimulatory substance produced by Escherichia coli ' Microbiology , vol 147 , no. 7 , pp. 1875-1885 . en
dc.identifier.issn 1465-2080
dc.identifier.other PURE: 130671
dc.identifier.other dspace: 2160/3642
dc.identifier.uri http://hdl.handle.net/2160/3642
dc.identifier.uri http://mic.sgmjournals.org/cgi/content/full/147/7/1875 en
dc.description Weichart, D. H., Kell, D. B. (2001). Characterization of an autostimulatory substance produced by Escherichia coli.  Microbiology, 147, (7), 1875-1885. Sponsorship: BBSRC en
dc.description.abstract The recovery of dilute populations of stationary phase cells of Escherichia coli was studied using an automatic growth analyser. The addition of 30% supernatant from 2-d-old stationary phase cells of the organism reproducibly shortened the apparent lag times by 22–57·5%, depending on the age of the inoculum. True lag times, as determined by colony counts, of stationary phase cells were reduced by supernatant addition by 41–62%. The growth-stimulating substance was characterized and partly purified from supernatants: the active material was shown to be dialysable, heat-stable, acid- and alkali-stable and protease-resistant. Extraction with ethyl acetate or ion-exchange resins was not successful, but the active material could be quantitatively extracted with ethanol after saturation with salt. It is concluded that the active substance is a small, non-proteinaceous, non-ionic organic molecule. Separation of extracts by HPLC indicated that the stimulatory substance is weakly hydrophobic and has retention times similar to those of uracil. So far, however, the exact chemical identity of the active substance has not been elucidated. en
dc.format.extent 11 en
dc.language.iso eng
dc.relation.ispartof Microbiology en
dc.title Characterization of an autostimulatory substance produced by Escherichia coli en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.contributor.institution Aberystwyth University en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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