Show simple item record Douglas B. en_US D. H. en_US 2009-11-26T09:46:11Z 2009-11-26T09:46:11Z 2001-07 en_US
dc.identifier.citation Kell , D B & Weichart , D H 2001 , ' Characterization of an autostimulatory substance produced by Escherichia coli ' Microbiology , vol 147 , no. 7 , pp. 1875-1885 . en_US
dc.identifier.other PURE: 130671 en_US
dc.identifier.other dspace: 2160/3642 en_US
dc.description.abstract The recovery of dilute populations of stationary phase cells of Escherichia coli was studied using an automatic growth analyser. The addition of 30% supernatant from 2-d-old stationary phase cells of the organism reproducibly shortened the apparent lag times by 22–57·5%, depending on the age of the inoculum. True lag times, as determined by colony counts, of stationary phase cells were reduced by supernatant addition by 41–62%. The growth-stimulating substance was characterized and partly purified from supernatants: the active material was shown to be dialysable, heat-stable, acid- and alkali-stable and protease-resistant. Extraction with ethyl acetate or ion-exchange resins was not successful, but the active material could be quantitatively extracted with ethanol after saturation with salt. It is concluded that the active substance is a small, non-proteinaceous, non-ionic organic molecule. Separation of extracts by HPLC indicated that the stimulatory substance is weakly hydrophobic and has retention times similar to those of uracil. So far, however, the exact chemical identity of the active substance has not been elucidated. en_US
dc.format.extent 11 en_US
dc.relation.ispartof Microbiology en_US
dc.title Characterization of an autostimulatory substance produced by Escherichia coli en_US
dc.contributor.pbl Aberystwyth University en_US
dc.contributor.pbl Institute of Biological, Environmental and Rural Sciences en_US

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