Changes in activities of acid invertase isoforms affect sugar accumulation and composition during ripening of tomato fruit

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dc.contributor.author Thomas, Barry J.
dc.contributor.author Husain, Sally E.
dc.contributor.author Nguyen-Quoc, B.
dc.contributor.author Kingston-Smith, Alison H.
dc.contributor.author N'tchobo, H.
dc.contributor.author Foyer, Christine H.
dc.date.accessioned 2009-11-26T12:00:29Z
dc.date.available 2009-11-26T12:00:29Z
dc.date.issued 2003-03
dc.identifier.citation Thomas , B J , Husain , S E , Nguyen-Quoc , B , Kingston-Smith , A H , N'tchobo , H & Foyer , C H 2003 , ' Changes in activities of acid invertase isoforms affect sugar accumulation and composition during ripening of tomato fruit ' Journal of Horticultural Science & Biotechnology , vol 78 , no. 2 , pp. 182-192 . en
dc.identifier.issn 1462-0316
dc.identifier.other PURE: 138490
dc.identifier.other dspace: 2160/3667
dc.identifier.uri http://hdl.handle.net/2160/3667
dc.identifier.uri http://www.jhortscib.org/Vol78/78_2/11.htm en
dc.description Husain, S. E., N'tchobo, H., Nguyen-Quoc, B., Kingston-Smith, A. H., Thomas, B. J., Foyer, C. H. (2003). Changes in activities of acid invertase isoforms affect sugar accumulation and composition during ripening of tomato fruit.  Journal of Horticultural Science and Biotechnology, 78, (2), 182-192. en
dc.description.abstract The observation that the wild, red fruited tomato Lycopersicon pimpinellifolium has higher soluble solids than the cultivated variety L. esculentum may be related to differential controls on fruit carbohydrate metabolism. We therefore investigated the role of acid invertase (AI) in determining fruit sugar composition in these two species. At all stages of fruit development, the majority of AI was localized in the vacuole with about 10&percent; of the total activity in the extra-cellular space. Four forms of soluble AI activity were identified. Isoform 1 persisted throughout development. Isoform 2 showed a strong relationship to total fruit sugar content in both species, suggesting that this isoform is the tiv1 gene product located in the vacuole, and that it plays a major role in determining fruit soluble solids. Isoform 3 was very high in L. pimpinellfolium but not in L. esculentum. We suggest that AI has a major role in determining fruit sugar accumulation in L. pimpinellifolium, but not in L. esculentum. Pulse-chase in vitro unloading experiments with [3h]-glucose showed that a substantial amount of [3h]-sucrose was present in the apoplast, suggesting an exchange of sugars between extra- and intra-cellular spaces. Hence ``futile cycles'' of sucrose synthesis and degradation involving apoplastic AI can occur during fruit development. en
dc.format.extent 11 en
dc.language.iso eng
dc.relation.ispartof Journal of Horticultural Science & Biotechnology en
dc.title Changes in activities of acid invertase isoforms affect sugar accumulation and composition during ripening of tomato fruit en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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