Show simple item record Jones, Alan M. Coimbra, Silvia Fath, Angelika Sottomayor, Mariana Thomas, Howard
dc.contributor.editor Schwartz, Lawrence M.
dc.contributor.editor Ashwell, Jonathan D. 2009-11-26T12:13:51Z 2009-11-26T12:13:51Z 2001-05-30
dc.identifier.citation Jones , A M , Coimbra , S , Fath , A , Sottomayor , M & Thomas , H 2001 , Programmed cell death assays for plants . in L M Schwartz & J D Ashwell (eds) , Apoptosis : Cell Death . vol. 66 , Methods in Cell Biology , Elsevier , pp. 437-451 . DOI: 10.1016/S0091-679X(01)66020-5 en
dc.identifier.isbn 9780125441650
dc.identifier.other PURE: 130757
dc.identifier.other PURE UUID: 0fb05dcf-f771-43d7-9972-b8dc68e9209c
dc.identifier.other dspace: 2160/3671
dc.description Chapter 19 en
dc.description.abstract Programmed cell death (PCD) is a critical part of normal development at all stages of the plant life cycle. This chapter discusses protocols for PCD assays. DNA degradation is an important diagnostic factor in programmed cell death, and cleavage of DNA in internucleosomal fragments of 180 bp is considered a hallmark of apoptosis. The ability to isolate undegraded high molecular weight DNA from viable tissue is a requirement for studying PCD-induced DNA degradation because it ensures that any DNA degradation observed is not an artifact of the isolation procedure. The barley aleurone layer is a model system to study hormonally induced PCD in plants. In this tissue, gibberellic acid (GA) induces PCD, whereas abscisic acid (ABA) prevents this program. The chapter describes a method for DNA isolation from barley aleurone cells that minimizes DNA degradation during the purification process. It has been shown to work well for high molecular weight DNA analysis in a variety of other tissue and cell preparations, for example, Arabidopsis thaliana leaves. en
dc.format.extent 15 en
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartof Apoptosis en
dc.relation.ispartofseries Methods in Cell Biology en
dc.rights en
dc.title Programmed cell death assays for plants en
dc.type /dk/atira/pure/researchoutput/researchoutputtypes/contributiontobookanthology/chapter en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en

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