Show simple item record Alan M. en_US Silvia en_US Angelika en_US Mariana en_US Howard en_US
dc.contributor.editor Lawrence M. en_US
dc.contributor.editor Jonathan D. en_US 2009-11-26T12:13:51Z 2009-11-26T12:13:51Z 2001-05-30 en_US
dc.identifier en_US
dc.identifier 9780125441650 en_US
dc.identifier.citation Jones , A M , Coimbra , S , Fath , A , Sottomayor , M & Thomas , H 2001 , ' Programmed cell death assays for plants ' . in L M Schwartz & J D Ashwell (eds) , Apoptosis : Cell Death . vol. 66 , Methods in Cell Biology , Elsevier , pp. 437-451 . , 10.1016/S0091-679X(01)66020-5 en_US
dc.identifier.other PURE: 130757 en_US
dc.identifier.other dspace: 2160/3671 en_US
dc.description.abstract Programmed cell death (PCD) is a critical part of normal development at all stages of the plant life cycle. This chapter discusses protocols for PCD assays. DNA degradation is an important diagnostic factor in programmed cell death, and cleavage of DNA in internucleosomal fragments of 180 bp is considered a hallmark of apoptosis. The ability to isolate undegraded high molecular weight DNA from viable tissue is a requirement for studying PCD-induced DNA degradation because it ensures that any DNA degradation observed is not an artifact of the isolation procedure. The barley aleurone layer is a model system to study hormonally induced PCD in plants. In this tissue, gibberellic acid (GA) induces PCD, whereas abscisic acid (ABA) prevents this program. The chapter describes a method for DNA isolation from barley aleurone cells that minimizes DNA degradation during the purification process. It has been shown to work well for high molecular weight DNA analysis in a variety of other tissue and cell preparations, for example, Arabidopsis thaliana leaves. en_US
dc.format.extent 15 en_US
dc.publisher Elsevier en_US
dc.relation.ispartof Apoptosis en_US
dc.relation.ispartofseries Methods in Cell Biology en_US
dc.title Programmed cell death assays for plants en_US
dc.contributor.pbl Institute of Biological, Environmental and Rural Sciences en_US

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