Programmed cell death assays for plants

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dc.contributor.author Jones, Alan M.
dc.contributor.author Coimbra, Silvia
dc.contributor.author Fath, Angelika
dc.contributor.author Sottomayor, Mariana
dc.contributor.author Thomas, Howard
dc.contributor.editor Schwartz, Lawrence M.
dc.contributor.editor Ashwell, Jonathan D.
dc.date.accessioned 2009-11-26T12:13:51Z
dc.date.available 2009-11-26T12:13:51Z
dc.date.issued 2001-05-30
dc.identifier.citation Jones , A M , Coimbra , S , Fath , A , Sottomayor , M & Thomas , H 2001 , ' Programmed cell death assays for plants ' . in L M Schwartz & J D Ashwell (eds) , Apoptosis : Cell Death . vol. 66 , Methods in Cell Biology , Academic Press , pp. 437-451 . en
dc.identifier.isbn 9780125441650
dc.identifier.other PURE: 130757
dc.identifier.other dspace: 2160/3671
dc.identifier.uri http://hdl.handle.net/2160/3671
dc.description Chapter 19 en
dc.description.abstract Programmed cell death (PCD) is a critical part of normal development at all stages of the plant life cycle. This chapter discusses protocols for PCD assays. DNA degradation is an important diagnostic factor in programmed cell death, and cleavage of DNA in internucleosomal fragments of 180 bp is considered a hallmark of apoptosis. The ability to isolate undegraded high molecular weight DNA from viable tissue is a requirement for studying PCD-induced DNA degradation because it ensures that any DNA degradation observed is not an artifact of the isolation procedure. The barley aleurone layer is a model system to study hormonally induced PCD in plants. In this tissue, gibberellic acid (GA) induces PCD, whereas abscisic acid (ABA) prevents this program. The chapter describes a method for DNA isolation from barley aleurone cells that minimizes DNA degradation during the purification process. It has been shown to work well for high molecular weight DNA analysis in a variety of other tissue and cell preparations, for example, Arabidopsis thaliana leaves. en
dc.format.extent 15 en
dc.language.iso eng
dc.publisher Academic Press
dc.relation.ispartof Apoptosis en
dc.relation.ispartofseries Methods in Cell Biology en
dc.title Programmed cell death assays for plants en
dc.type Text en
dc.type.publicationtype Book chapter en
dc.identifier.doi http://dx.doi.org/10.1016/S0091-679X(01)66020-5
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en


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