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dc.contributor.author John F. en_US
dc.contributor.author Olga A. en_US
dc.contributor.author Christopher J. en_US
dc.contributor.author A. Deri en_US
dc.contributor.author Joe A. en_US
dc.date.accessioned 2009-11-26T15:57:40Z
dc.date.available 2009-11-26T15:57:40Z
dc.date.issued 2001 en_US
dc.identifier http://dx.doi.org/10.1111/j.1744-7348.2001.tb00082.x en_US
dc.identifier.citation Farrar , J F , Koroleva , O A , Pollock , C J , Tomos , A D & Gallagher , J A 2001 , ' Carbon allocation and sugar status in individual cells of barley leaves affects expression of sucrose:fructan 6-fructosyltransferase gene ' Annals of Applied Biology , pp. 27-32 . , 10.1111/j.1744-7348.2001.tb00082.x en_US
dc.identifier.other PURE: 130337 en_US
dc.identifier.other dspace: 2160/3695 en_US
dc.identifier.uri http://hdl.handle.net/2160/3695
dc.description.abstract Heterogeneity of function in plant tissues requires precise regulation of metabolic systems controlling resource allocation at the level of single cells. Differential distribution of metabolites between epidermis, mesophyll and parenchymatous bundle sheath (PBS) cells has already been demonstrated in barley (Hordeum vulgare L.) source leaves and partitioning of sugars in barley leaves has been proven to be highly cell-type specific. Carbohydrate metabolism of barley leaves induced to accumulate sucrose and fructans was investigated further at the individual cell level, using single cell sampling and analysis techniques. A novel approach using reverse transcription followed by polymerase chain reaction (RT-PCR) was used to analyse expression of the enzyme Sucrose: Fructan 6-Fructosyltransferase (6-SFT) in individual cells of barley leaves. Accumulation of sucrose and the synthesis of fructans were induced by reducing export of sugars from leaves (root cooling), by increasing photosynthesis (high photon fluence rates), or by supplying sugars exogenously. In all cases there was a positive qualitative correlation between the presence of 6-SFT transcripts and accumulation of fructan and sucrose in both mesophyll and PBS cells. en_US
dc.format.extent 6 en_US
dc.relation.ispartof Annals of Applied Biology en_US
dc.title Carbon allocation and sugar status in individual cells of barley leaves affects expression of sucrose:fructan 6-fructosyltransferase gene en_US
dc.contributor.pbl Aberystwyth University en_US
dc.contributor.pbl Institute of Biological, Environmental and Rural Sciences en_US


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