Show simple item record Rose, Michael T. Hagino, A. Yonekura, S. Aso, Hisashi Ozutsumi, K. Obara, Y. Komatsu, T. 2009-11-30T09:59:59Z 2009-11-30T09:59:59Z 2009-11-30
dc.identifier.citation Rose , M T , Hagino , A , Yonekura , S , Aso , H , Ozutsumi , K , Obara , Y & Komatsu , T 2009 , ' In vitro differentiation of a cloned bovine mammary epithelial cell ' Unknown Journal , pp. 345-355 . en
dc.identifier.other PURE: 140196
dc.identifier.other dspace: 2160/3716
dc.description Rose, M. T., Aso, H., Yonekura, S., Komatsu, T., Hagino, A., Ozutsumi, K., Obara, Y. (2002). In vitro differentiation of a cloned bovine mammary epithelial cell.  Journal of Dairy Research, 69, (3), 345-355 en
dc.description.abstract The aim of the study was to establish in vitro a bovine mammary epithelial cell (MEC) clone, able to respond to mitogenic growth factors and to lactogenic hormones. Mammary tissue from a 200-d pregnant Holstein cow was used as a source of MEC, from which a clone was established through a process of limiting dilution. When plated on plastic, the cells assumed a monolayer, cobblestone, epithelial-like morphology, with close contact between cells. Inclusion of IGF-1 and EGF in the media significantly increased the number of cells 5 d after plating. All cells stained strongly for cytokeratin and moderately for vimentin at young and old passage stages, indicating the epithelial nature of this cell clone. When the cells were plated at a high density on a thin layer of a commercial extracellular matrix preparation (Matrigel), lobular, alveoli-like structures developed within approximately 5 d, with a clearly visible lumen. When cells were plated onto Matrigel in differentiation media (containing lactogenic hormones), detectable quantities of ¿-casein were present in the media and particularly on the lumen side of the structures. Omission of one of the lactogenic hormones (insulin, prolactin or hydrocortisone) reduced ¿-casein release to the limit of detection of the assay used. Lactoferrin was also produced when the cells were plated on Matrigel, again principally on the lumen side of the lobules, though this was independent of the lactogenic hormones. By passage 40, the cells had senesced, and it was not possible to induce ¿-casein or lactoferrin production. This study notes the establishment of a functional bovine mammary epithelial cell clone, which is responsive to mitogenic and lactogenic hormones and an extracellular matrix. en
dc.format.extent 11 en
dc.language.iso eng
dc.relation.ispartof Unknown Journal en
dc.title In vitro differentiation of a cloned bovine mammary epithelial cell en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en

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