A cytokinin-binding 70-kD protein is localized predominantly in the root meristem

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dc.contributor.author Shepelyakovskaya, A. O.
dc.contributor.author Teplova, I. R.
dc.contributor.author Veselov, D. S.
dc.contributor.author Burkhanova, E. A.
dc.contributor.author Bosiev, K. M.
dc.contributor.author Laman, A. G.
dc.contributor.author Vasil'eva, V. S.
dc.contributor.author Kudoyarova, G. R.
dc.contributor.author Hall, Michael A.
dc.contributor.author Brovko, F. A.
dc.contributor.author Kulaeva, O. N.
dc.date.accessioned 2009-12-10T09:09:33Z
dc.date.available 2009-12-10T09:09:33Z
dc.date.issued 2002-01
dc.identifier.citation Shepelyakovskaya , A O , Teplova , I R , Veselov , D S , Burkhanova , E A , Bosiev , K M , Laman , A G , Vasil'eva , V S , Kudoyarova , G R , Hall , M A , Brovko , F A & Kulaeva , O N 2002 , ' A cytokinin-binding 70-kD protein is localized predominantly in the root meristem ' Russian Journal of Plant Physiology , vol 49 , no. 1 , pp. 99-106 . en
dc.identifier.issn 1608-3407
dc.identifier.other PURE: 132146
dc.identifier.other dspace: 2160/3795
dc.identifier.uri http://hdl.handle.net/2160/3795
dc.description Shepelyakovskaya, A. O., Teplova, I. R., Veselov, D. S., Burkhanova, E. A., Bosiev, K. M., Laman, A. G., Vasil'eva, V. S., Kudoyarova, G. R., Hall, M. A., Brovko, F. A., Kulaeva, O. N. (2002). A cytokinin-binding 70-kD protein is localized predominantly in the root meristem. Russian Journal of Plant Physiology, 49, (1), 99-106. Zea mays - meristem - receptor - cytokinin - cytokinin-binding protein Sponsorship: Russian Foundation for Basic Research, projects nos. 99-04-49166, 99-04-49042, 00-15-97899, 01-04-48506 / INTAS grant N 99-0120 en
dc.description.abstract The contents of cytokinins (zeatin, zeatin riboside, zeatin nucleotide, and zeatin N9-glucoside), total protein, and a 70-kD cytokinin-binding protein (CBP70) were estimated in the root meristem and elongation zones of four-day-old etiolated maize seedlings. Earlier, it was shown that CBP70 is a cytokinin receptor involved in the hormone-induced activation of transcriptional elongation. A pair of monoclonal anti-CBP70 antibodies were selected; this permitted us to use a sandwich-type immunoenzyme analysis in combination with the streptovidin–biotin system, and this improved substantially the sensitivity of the CBP70 measurement. As calculated per cell, the elongating cells were enriched in all cytokinin forms, especially in transport cytokinins (zeatin riboside and zeatin nucleotide) as compared to the meristem cells, but the meristemic cell contained three times less total protein and two times more CBP70 than the cell in the elongation zone. The CBP70 concentration per milligram of total protein was six times higher in the cytosol of the meristemic cell as compared to the elongation zone. Predominant localization of the CBP70 in the meristem implies its involvement in the cytokinin control of cell division. en
dc.format.extent 8 en
dc.language.iso eng
dc.relation.ispartof Russian Journal of Plant Physiology en
dc.subject Zea mays en
dc.subject meristem en
dc.subject receptor en
dc.subject cytokinin en
dc.subject cytokinin-binding protein en
dc.title A cytokinin-binding 70-kD protein is localized predominantly in the root meristem en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1023/A:1013772530375
dc.contributor.institution Aberystwyth University en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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