Show simple item record Begley, P. Sims, Ian M. Cairns, Andrew J. 2009-12-15T15:24:27Z 2009-12-15T15:24:27Z 2002
dc.identifier.citation Begley , P , Sims , I M & Cairns , A J 2002 , ' The structure of starch from seeds and leaves of the fructan- accumulating ryegrass, Lolium temulentum ' Journal of Plant Physiology , vol 159 , no. 3 , pp. 221-230 . DOI: 10.1078/0176-1617-00685 en
dc.identifier.issn 1618-1328
dc.identifier.other PURE: 134291
dc.identifier.other PURE UUID: 1b86414b-c9e1-40ea-8645-07a702eb7885
dc.identifier.other dspace: 2160/3831
dc.identifier.other DSpace_20121128.csv: row: 2968
dc.identifier.other Scopus: 0036203521
dc.description Cairns, A. J., Begley, P., Sims, I. M. (2002). The structure of starch from seeds and leaves of the fructan- accumulating ryegrass, Lolium temulentum.   Journal of Plant Physiology, 159, (3), 221-230 Sponsorship: BBSRC en
dc.description.abstract Starch granules isolated from leaves and seeds of Lolium temulentum L. were roughly spherical with an irregular perimeter having mean diameters of 1.7 and 4.1 μm respectively. On a dry mass basis, 47 % of the seed and maximally 4 % of the leaf were starch. Starch structures were deduced from a combination of data obtained using acid-hydrolysis/Smith degradation, glycosyl-linkage analysis/GC-MS, iodine complex formation, isoamylase digestion, high performance size exclusion chromatography and high performance anion exchange chromatography/pulsed amperometry. The mean degree of polymerisation (DP) of seed starch was 4800 (Mr = 778 kDa) and consisted entirely of a highly branched, amylopectin-like polymer with an average chain length of DP = 46 ± 6 and an average of 104 chains per molecule. Leaf starch was 98.8 % by mass of a highly-branched, amylopectin-like polymer of DP = 5850 (Mr 947 kDa) containing on average, 136 chains of mean DP = 43 ± 6. The remaining 1.2 % of the leaf starch was isoamylase-resistant, of high Mr (5850 kDa) and was designated amylose. Both starches were unusual in that the chain lengths were longer by a factor of 2, than is normal for amylopectins and also because they contained little or no long 1,4-linked chains, as determined by isoamylase digestion. HPAEC of isoamylase fragments from leaf starch revealed a bi-modal distribution of branch chain lengths which was not evident in the seed starch. With the exceptions of this bi-modality and the trace of amylose in the leaf starch, the structure of the starches from the two organs were very similar. Seed starch exhibited an anomalous reaction with iodine. The chromogen was deep blue with a λmax = 608 nm, indicative of the presence of long amylose chains. On digestion with isoamylase, all of the mass of starch was converted to low molecular mass with an iodine λmax shifted to 567 nm. Hence, short 1,4-linked glucan chains which were contiguous through 1,6-linkages formed blue chromogens and can give the appearance of amylose. en
dc.format.extent 10 en
dc.language.iso eng
dc.relation.ispartof Journal of Plant Physiology en
dc.rights en
dc.subject carbohydrate en
dc.subject feed quality en
dc.subject grass forage en
dc.subject polysaccharide en
dc.subject ruminant nutrition en
dc.title The structure of starch from seeds and leaves of the fructan- accumulating ryegrass, Lolium temulentum en
dc.type /dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en

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