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dc.contributor.author K. en_US
dc.contributor.author Nigel J. en_US
dc.contributor.author David C. en_US
dc.contributor.author Diane E. en_US
dc.contributor.author T. en_US
dc.contributor.author L. M. en_US
dc.contributor.author M. R. en_US
dc.contributor.author Steven L. en_US
dc.date.accessioned 2009-12-15T16:39:35Z
dc.date.available 2009-12-15T16:39:35Z
dc.date.issued 2002 en_US
dc.identifier http://dx.doi.org/10.1042/BJ20011380 en_US
dc.identifier.citation Fowler , K , Manning , N J , Lamb , D C , Kelly , D E , Kieser , T , Podust , L M , Waterman , M R & Kelly , S L 2002 , ' Sterol 14alpha-demethylase activity in Streptomyces coelicolor A3(2) is associated with an unusual member of the CYP51 gene family ' Biochemical Journal , vol 364 , no. 2 , pp. 555-562 . , 10.1042/BJ20011380 en_US
dc.identifier.other PURE: 132476 en_US
dc.identifier.other dspace: 2160/3840 en_US
dc.identifier.uri http://hdl.handle.net/2160/3840
dc.description.abstract The annotation of the genome sequence of Streptomyces coelicolor A3(2) revealed a cytochrome P450 (CYP) resembling various sterol 14a-demethylases (CYP51). The putative CYP open reading frame (SC7E4.20) was cloned with a tetrahistidine tag appended to the C-terminus and expressed in Escherichia coli. Protein purified to electrophoretic homogeneity was observed to bind the 14-methylated sterols lanosterol and 24-methylene-24,25-dihydrolanosterol (24-MDL). Reconstitution experiments with E. coli reductase partners confirmed activity in 14a-demethylation for 24-MDL, but not lanosterol. An S. coelicolor A3(2) mutant containing a transposon insertion in the CYP51 gene, which will abolish synthesis of the functional haemoprotein, was isolated as a viable strain, the first time a CYP51 has been identified as non-essential. The role of this CYP in bacteria is intriguing. No sterol product was detected in non-saponifiable cell extracts of the parent S. coelicolor A3(2) strain or of the mutant. S. coelicolor A3(2) CYP51 contains very few of the conserved CYP51 residues and, even though it can catalyse 14a-demethylation, it probably has another function in Streptomyces. We propose that it is a member of a new CYP51 subfamily. en_US
dc.format.extent 8 en_US
dc.relation.ispartof Biochemical Journal en_US
dc.title Sterol 14alpha-demethylase activity in Streptomyces coelicolor A3(2) is associated with an unusual member of the CYP51 gene family en_US
dc.contributor.pbl Aberystwyth University en_US
dc.contributor.pbl Institute of Biological, Environmental and Rural Sciences en_US


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