Flow-injection electrospray ionization mass spectrometry of crude cell extracts for high-throughput bacterial identification

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dc.contributor.author Vaidyanathan, Seetharaman
dc.contributor.author Kell, Douglas B.
dc.contributor.author Goodacre, Royston
dc.date.accessioned 2009-12-23T10:41:32Z
dc.date.available 2009-12-23T10:41:32Z
dc.date.issued 2002
dc.identifier.citation Vaidyanathan , S , Kell , D B & Goodacre , R 2002 , ' Flow-injection electrospray ionization mass spectrometry of crude cell extracts for high-throughput bacterial identification ' Journal of the Americal Society for Mass Spectrometry , vol 13 , no. 2 , pp. 118-128 . , 10.1016/S1044-0305(01)00339-7 en
dc.identifier.issn 1879-1123
dc.identifier.other PURE: 132845
dc.identifier.other dspace: 2160/3912
dc.identifier.uri http://hdl.handle.net/2160/3912
dc.description Vaidyanathan, S., Kell, D. B., Goodacre, R. (2002). Flow-injection electrospray ionization mass spectrometry of crude cell extracts for high-throughput bacterial identification. Journal of the Americal Society for Mass Spectrometry, 13, (2), 118-128 Sponsorship: BBSRC en
dc.description.abstract Flow-injection electrospray ionization mass spectrometry (FI-ESI-MS) of unfractionated cell-free extracts obtained from bacterial cells suspended in a solvent mixture was investigated as a rapid analytical method for reproducible, high-throughput bacterial identification. Five bacterial strains (two Escherichia coli, two Bacillus spp. and one Brevibacillus laterosporus) were studied in this investigation. Axenically grown bacterial cells were suspended in an acidic organic solvent and the cell-free extract was sequentially injected into a solvent flow stream that was sprayed into the ionization chamber of the ESI-MS. The spectra produced contained reproducible information, which was useful for discriminating between the bacteria. Tandem mass spectrometry was used to characterize further the peaks, and at least three classes of macromolecules, namely phospholipids, glycolipids, and proteins, were found to contribute most to the spectral information. Bacterial extracts stored under different conditions gave very similar mass spectra for each of the five bacterial strains, indicating that the extracts were stable even at room temperature for up to 24 h, with no loss of information content, which has obvious implications for automated high-throughput analysis. An analysis of the components of the extracting solvent mixture and their effects on the spectral information showed that acetonitrile contributes most significantly to the extraction process and hence to the information content of the spectra. en
dc.format.extent 11 en
dc.language.iso eng
dc.relation.ispartof Journal of the Americal Society for Mass Spectrometry en
dc.title Flow-injection electrospray ionization mass spectrometry of crude cell extracts for high-throughput bacterial identification en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1016/S1044-0305(01)00339-7
dc.contributor.institution Aberystwyth University en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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