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dc.contributor.author Prewer, W.
dc.contributor.author Marshall, Athole H.
dc.contributor.author Carlile, E.
dc.contributor.author Michaelson-Yeates, Terence P. T.
dc.contributor.author Abberton, Michael T.
dc.contributor.author Bowen, Charlotte
dc.date.accessioned 2010-01-05T09:38:39Z
dc.date.available 2010-01-05T09:38:39Z
dc.date.issued 2010-01-05
dc.identifier.citation Prewer , W , Marshall , A H , Carlile , E , Michaelson-Yeates , T P T , Abberton , M T & Bowen , C 2010 , ' Bulked segregant AFLP analysis to identify markers for the introduction of the rhizomatous habit from Trifolium ambiguum into T-repens (white clover) ' Unknown Journal , pp. 217-222 . en
dc.identifier.other PURE: 145100
dc.identifier.other dspace: 2160/3933
dc.identifier.uri http://hdl.handle.net/2160/3933
dc.description Abberton, M. T., Michaelson-Yeates, T. P. T., Bowen C., Marshall, A. H., Prewer, W., Carlile, E. (2003). Bulked segregant AFLP analysis to identify markers for the introduction of the rhizomatous habit from Trifolium ambiguum into T-repens (white clover).   Euphytica, 134, (2), 217-222. en
dc.description.abstract Bulked segregant analysis (BSA) using the technique of amplified fragment length polymorphism (AFLPTM) was carried out on backcross hybrids between the important pasture species white clover and its relative Trifolium ambiguum. The introgression of the rhizomatous trait fromTrifolium ambiguum, previously shown to be associated with enhanced drought tolerance but which takes eighteen months to manifest itself, was analysed in a series of third generation backcross families with white clover as the recurrent parent. Eleven families differed in the proportions of plants classified according to rhizome content. Three of these families were selected for BSA-AFLP based on the creation of separate DNA pools from plants with large amounts of rhizomes or no rhizomes. Following an initial screen with119 primer combinations, 53 combinations were selected on the basis of the number of clear, reproducible polymorphisms they produced. Polymorphic bands were found that distinguished between rhizomatous and non-rhizomatous bulks in the three families. However, only one polymorphic band was found that unambiguously reflected the presence of the rhizomatous trait in individual plants in these families. This polymorphic band was also shown to be present in the BC2 parent, the BC1 and the original T. ambiguum parent. Given the aneuploid nature of the BC2 plants this band would appear to be a chromosome-specific marker tightly linked to the locus controlling rhizome development. The identification of a consistent marker for the rhizomatous trait opens up the possibility of applying marker assisted introgression and thereby facilitating the rapid incorporation of this trait into diverse germplasm. en
dc.format.extent 6 en
dc.language.iso eng
dc.relation.ispartof Unknown Journal en
dc.title Bulked segregant AFLP analysis to identify markers for the introduction of the rhizomatous habit from Trifolium ambiguum into T-repens (white clover) en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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