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dc.contributor.author Kaprelyants, Arseny S.
dc.contributor.author Mukamolova, Galina V.
dc.contributor.author Young, Michael
dc.contributor.author Turapov, Obolbek A.
dc.contributor.author Young, Danielle I.
dc.contributor.author Kell, Douglas B.
dc.date.accessioned 2010-01-05T16:37:56Z
dc.date.available 2010-01-05T16:37:56Z
dc.date.issued 2002-10-31
dc.identifier.citation Kaprelyants , A S , Mukamolova , G V , Young , M , Turapov , O A , Young , D I & Kell , D B 2002 , ' A family of autocrine growth factors in Mycobacterium tuberculosis ' Molecular Microbiology , vol 46 , no. 3 , pp. 623-635 . , 10.1046/j.1365-2958.2002.03184.x en
dc.identifier.issn 0950-382X
dc.identifier.other PURE: 134031
dc.identifier.other dspace: 2160/3941
dc.identifier.uri http://hdl.handle.net/2160/3941
dc.description Mukamolova, G. V., Turapov, O. A., Young, D. I., Kaprelyants, A. S., Kell, D. B., Young, M. (2002). A family of autocrine growth factors in Mycobacterium tuberculosis.   Molecular Microbiology, 46, (3), 623-635. Sponsorship: BBSRC; Russian Foundation for Basic Research; WHO Global Programme for Vaccines and Immunization; Wellcome Trust en
dc.description.abstract Mycobacterium tuberculosis and its close relative, Mycobacterium bovis (BCG) contain five genes whose predicted products resemble Rpf from Micrococcus luteus. Rpf is a secreted growth factor, active at picomolar concentrations, which is required for the growth of vegetative cells in minimal media at very low inoculum densities, as well as the resuscitation of dormant cells. We show here that the five cognate proteins from M. tuberculosis have very similar characteristics and properties to those of Rpf. They too stimulate bacterial growth at picomolar (and in some cases, subpicomolar) concentrations. Several lines of evidence indicate that they exert their activity from an extra-cytoplasmic location, suggesting that they are also involved in intercellular signalling. The five M. tuberculosis proteins show cross-species activity against M. luteus, Mycobacterium smegmatis and M. bovis (BCG). Actively growing cells of M. bovis (BCG) do not respond to these proteins, whereas bacteria exposed to a prolonged stationary phase do. Affinity-purified antibodies inhibit bacterial growth in vitro, suggesting that sequestration of these proteins at the cell surface might provide a means to limit or even prevent bacterial multiplication in vivo. The Rpf family of bacterial growth factors may therefore provide novel opportunities for preventing and controlling mycobacterial infections. en
dc.format.extent 13 en
dc.language.iso eng
dc.relation.ispartof Molecular Microbiology en
dc.title A family of autocrine growth factors in Mycobacterium tuberculosis en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1046/j.1365-2958.2002.03184.x
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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